Development of size-customized hepatocarcinoma spheroids as a potential drug testing platform using a sacrificial gelatin microsphere system

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

15 Scopus Citations
View graph of relations

Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)644-649
Journal / PublicationMaterials Science and Engineering C
Volume63
Online published16 Mar 2016
Publication statusPublished - 1 Jun 2016
Externally publishedYes

Abstract

Sacrificial gelatin microspheres can be developed as a cell delivery vehicle for non-anchorage dependent cells - its incorporation into a macroscopic scaffold system not only allows the cells to be cultured in suspension within cavities left behind by the sacrificial material, it also allows scaffold-free tissue development to be confined within the cavities. In this study, dense and highly viable hepatocarcinoma spheroids were developed by means of encapsulation in sacrificial gelatin microspheres produced via a simple water-in-oil emulsion technique. By initial selection of microsphere size and distribution, spheroid size can be controlled for various applications such as uniform tumor spheroids as a reproducible three-dimensional drug screening and testing platform that better mimics the in vivo nature of tumors (instead of conventional monolayer culture), as this study has suggested as a proof-of-concept with chemotherapy drug Doxorubicin. © 2016 Elsevier B.V. All rights reserved.

Research Area(s)

  • 3D drug testing model, Cell encapsulation, Sacrificial microsphere, Tumor spheroid formation

Citation Format(s)

Development of size-customized hepatocarcinoma spheroids as a potential drug testing platform using a sacrificial gelatin microsphere system. / Leong, Wenyan; Kremer, Antje; Wang, Dong-An.
In: Materials Science and Engineering C, Vol. 63, 01.06.2016, p. 644-649.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review