Development of immunoassays for protein analysis on nanobioarray chips

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)12_Chapter in an edited book (Author)peer-review

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Author(s)

Detail(s)

Original languageEnglish
Title of host publicationMicrofluidic Devices for Biomedical Applications
PublisherElsevier Inc.
Pages445-464
ISBN (Print)9780857096975
Publication statusPublished - Oct 2013
Externally publishedYes

Abstract

Immunoassays have been utilized as a method to detect secreted proteins such as antibodies or cytokines. These soluble proteins in blood or serum allow for clinical diagnostics of diseases ranging from hepatitis, autoimmune disorders, and Alzheimer's to cancer. Immunoassays can be homogeneous or heterogeneous, with heterogeneous immunoassay (e.g. enzyme-linked immunosorbent assay (ELISA)) being much more common because the unbound proteins are easily washed away from the solid substrate. The need for sample volumes in excess of 10 μL in ELISA poses a restriction to the detection of antibodies and cytokines, usually found in low concentrations and low volumes in clinical samples. This necessitates the development of nanoliter-volume liquid handling, and high detection sensitivity of low- volume and low-concentration samples. The recent development of the nanobioarray chip technology will address these needs. © 2013 Woodhead Publishing Limited All rights reserved..

Research Area(s)

  • Antibody, Chemiluminescence, Covalent immobilization, Cytokine, Enzyme-linked immunosorbent assay, Fluorescence, Heterogeneous immunoassay, Mosaic array

Bibliographic Note

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Citation Format(s)

Development of immunoassays for protein analysis on nanobioarray chips. / Lee, J.; Li, P. C.H.

Microfluidic Devices for Biomedical Applications. Elsevier Inc., 2013. p. 445-464.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)12_Chapter in an edited book (Author)peer-review