TY - JOUR
T1 - Development of HPLC fingerprint for species differentiation and quality assessment of Rhizoma Smilacis Glabrae
AU - Zhang, Qing-Feng
AU - Cheung, Hon-Yeung
AU - Zeng, Ling-Bin
PY - 2013/1
Y1 - 2013/1
N2 - Rhizoma Smilacis Glabrae (RSG) is a commonly used herbal material in functional food and Traditional Chinese Medicine. A HPLC chromatographic fingerprint was developed for its quality control and species differentiation. Nine peaks were found in the chromatogram of RSG and all these peaks were identified by diode array detection and electrospray ionization-MS/MS: 5-O-caffeoylshikimic acid, taxifolin, engeletin, isoengeletin, trans-resveratrol, astilbin and its three stereoisomers. Six of these constituents were consistently found in 18 batches of samples. The standard fingerprint of RSG was generated by mean simulation of all tested samples. Using the standard fingerprint, RSG could be easily differentiated from Rhizoma Smilacis Chinae and Rhizoma Heterosmilacis, the two species that can be confused with RSG. © 2012 The Japanese Society of Pharmacognosy and Springer.
AB - Rhizoma Smilacis Glabrae (RSG) is a commonly used herbal material in functional food and Traditional Chinese Medicine. A HPLC chromatographic fingerprint was developed for its quality control and species differentiation. Nine peaks were found in the chromatogram of RSG and all these peaks were identified by diode array detection and electrospray ionization-MS/MS: 5-O-caffeoylshikimic acid, taxifolin, engeletin, isoengeletin, trans-resveratrol, astilbin and its three stereoisomers. Six of these constituents were consistently found in 18 batches of samples. The standard fingerprint of RSG was generated by mean simulation of all tested samples. Using the standard fingerprint, RSG could be easily differentiated from Rhizoma Smilacis Chinae and Rhizoma Heterosmilacis, the two species that can be confused with RSG. © 2012 The Japanese Society of Pharmacognosy and Springer.
KW - Fingerprint
KW - HPLC/DAD/ESI-MS/MS
KW - Rhizoma Smilacis Glabrae
KW - Species differentiation
UR - http://www.scopus.com/inward/record.url?scp=84871266414&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84871266414&origin=recordpage
U2 - 10.1007/s11418-012-0648-9
DO - 10.1007/s11418-012-0648-9
M3 - RGC 21 - Publication in refereed journal
C2 - 22382863
SN - 1340-3443
VL - 67
SP - 207
EP - 211
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
IS - 1
ER -