Development of dual-component protein microparticles in all-aqueous systems for biomedical applications

Yi Deng; Qingming Ma; Hao Yuan; Galen Chit Lum; Ho Cheung Shum

doi:10.1039/c8tb03074j

Development of dual-component protein microparticles in all-aqueous systems for biomedical applications

Yi Deng
, Qingming Ma
, Hao Yuan
, Galen Chit Lum
, Ho Cheung Shum^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

20 Citations (Scopus)

Abstract

Protein microparticles assisted by an emulsion droplet template have shown great promise in drug/cell delivery and tissue engineering, as well as diagnosis and treatment of diseases. However, the usage of non-aqueous solvents involved in the oil-containing emulsion and their single-component nature severely hamper their use in medical applications. To address these limitations, here we present a facile strategy to fabricate dual-component protein (DCP) microparticles via the microfluidic electrospray technique. Due to the affinity partitioning properties of the all-aqueous system (AAS), electrostatic complexation takes place between the two oppositely charged proteins to form dual-component protein-protein structures in the all-aqueous droplets. We demonstrate that hemoglobin-bovine serum albumin (Hb-BSA) DCP microparticles possess characteristics resembling those of natural red blood cells, including remarkable softness and the ability to pass through narrow channels. Moreover, in vitro results show that other hemoglobin-immunoglobulin (Hb-BSA) DCP microparticles exhibit good cytocompatibility towards human stem cells. Accordingly, this work provides a novel engineering strategy using all-aqueous droplets as a template to design new biocompatible DCP microparticles for biomedical applications. © The Royal Society of Chemistry 2019.

Original language	English
Pages (from-to)	3059-3065
Journal	Journal of Materials Chemistry B
Volume	7
Issue number	19
DOIs	https://doi.org/10.1039/c8tb03074j
Publication status	Published - Jan 2019
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

Y. Deng and Q. Ma contributed equally to this work. This study was supported by the General Research Fund (No. 17304017, 17304514, 17306315 and 17329516) from the Research Grants Council of HK, the Fund (91434202) from the National Natural Science Foundation of China (21476189/B060201, 91434202, and 81801848), the Seed Funding Program for Basic Research (No. 201711159249, 201611159205 and 201511159280), Seed Fund for Translational and Applied Research (201711160016) and Platform Technology Funding from HKU, Sichuan Science and Technology Program (2017FZ0046, 2018JZ0026), China Postdoctoral Science Foundation (2017M610600), the Fundamental Research Funds for the Central Universities (2017SCU12016), the International Science and Technology Cooperation Funding of Chengdu (2017-GH02-00025-HZ), and the Hong Kong Scholars Program. We also thank Dr Michael K. T. To and Miss. D. Ao for supplying the red blood cells.

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title = "Development of dual-component protein microparticles in all-aqueous systems for biomedical applications",

abstract = "Protein microparticles assisted by an emulsion droplet template have shown great promise in drug/cell delivery and tissue engineering, as well as diagnosis and treatment of diseases. However, the usage of non-aqueous solvents involved in the oil-containing emulsion and their single-component nature severely hamper their use in medical applications. To address these limitations, here we present a facile strategy to fabricate dual-component protein (DCP) microparticles via the microfluidic electrospray technique. Due to the affinity partitioning properties of the all-aqueous system (AAS), electrostatic complexation takes place between the two oppositely charged proteins to form dual-component protein-protein structures in the all-aqueous droplets. We demonstrate that hemoglobin-bovine serum albumin (Hb-BSA) DCP microparticles possess characteristics resembling those of natural red blood cells, including remarkable softness and the ability to pass through narrow channels. Moreover, in vitro results show that other hemoglobin-immunoglobulin (Hb-BSA) DCP microparticles exhibit good cytocompatibility towards human stem cells. Accordingly, this work provides a novel engineering strategy using all-aqueous droplets as a template to design new biocompatible DCP microparticles for biomedical applications. {\textcopyright} The Royal Society of Chemistry 2019.",

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AU - Shum, Ho Cheung

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AB - Protein microparticles assisted by an emulsion droplet template have shown great promise in drug/cell delivery and tissue engineering, as well as diagnosis and treatment of diseases. However, the usage of non-aqueous solvents involved in the oil-containing emulsion and their single-component nature severely hamper their use in medical applications. To address these limitations, here we present a facile strategy to fabricate dual-component protein (DCP) microparticles via the microfluidic electrospray technique. Due to the affinity partitioning properties of the all-aqueous system (AAS), electrostatic complexation takes place between the two oppositely charged proteins to form dual-component protein-protein structures in the all-aqueous droplets. We demonstrate that hemoglobin-bovine serum albumin (Hb-BSA) DCP microparticles possess characteristics resembling those of natural red blood cells, including remarkable softness and the ability to pass through narrow channels. Moreover, in vitro results show that other hemoglobin-immunoglobulin (Hb-BSA) DCP microparticles exhibit good cytocompatibility towards human stem cells. Accordingly, this work provides a novel engineering strategy using all-aqueous droplets as a template to design new biocompatible DCP microparticles for biomedical applications. © The Royal Society of Chemistry 2019.

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Development of dual-component protein microparticles in all-aqueous systems for biomedical applications

Abstract

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