Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine faeces

T. La; A. M. Collins; N. D. Phillips; A. Oksa; D. J. Hampson

doi:10.1111/j.1472-765X.2005.01831.x

Author(s)

T. La
A. M. Collins
N. D. Phillips
A. Oksa
D. J. Hampson

Detail(s)

Original language	English
Pages (from-to)	284-288
Journal / Publication	Letters in Applied Microbiology
Volume	42
Issue number	3
Online published	25 Jan 2006
Publication status	Published - Mar 2006
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1111/j.1472-765X.2005.01831.x Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-33645215615&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(346500e4-9ad6-4a29-bb88-27f6680bdfee).html

Abstract

Aims: To develop an assay to simultaneously detect Lawsonia intracellularis, Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig faeces.

Methods and Results: A multiplex-polymerase chain reaction (M-PCR) was designed to amplify a 655-base pair (bp) portion of the L. intracellularis 16S rRNA gene, a 354-bp portion of the B. hyodysenteriae NADH oxidase gene, and a 823-bp portion of the B. pilosicoli 16S rRNA gene. Specificity was assessed using 80 strains of Brachyspira spp. and 30 other enteric bacteria. Bacterial DNA was extracted from faeces using the QIAamp^® DNA Stool Mini Kit. The M-PCR was tested in parallel with culture and/or PCR on 192 faecal samples from eight piggeries. Faeces also were seeded with known cell concentrations of the three pathogenic species, and the limits of detection of the M-PCR tested. The M-PCR was specific, with limits of detection of 10²–10³ cells of the respective species per gram of faeces.

Conclusions: The M-PCR is a rapid, sensitive and specific test for detecting three important enteric bacterial pathogens of pigs.

Significance and Impact of the Study: The availability of a new diagnostic M-PCR will allow rapid detection and control of three key porcine enteric pathogens.

Research Area(s)

Brachyspira, Diarrhoea, Lawsonia, Multiplex-PCR, Pigs

Citation Format(s)

[ RIS ] [ BibTeX ]

Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine faeces. / La, T.; Collins, A. M.; Phillips, N. D. et al.
In: Letters in Applied Microbiology, Vol. 42, No. 3, 03.2006, p. 284-288.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

La, T, Collins, AM, Phillips, ND, Oksa, A & Hampson, DJ 2006, 'Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine faeces', Letters in Applied Microbiology, vol. 42, no. 3, pp. 284-288. https://doi.org/10.1111/j.1472-765X.2005.01831.x

La, T., Collins, A. M., Phillips, N. D., Oksa, A., & Hampson, D. J. (2006). Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine faeces. Letters in Applied Microbiology, 42(3), 284-288. https://doi.org/10.1111/j.1472-765X.2005.01831.x

La T, Collins AM, Phillips ND, Oksa A, Hampson DJ. Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine faeces. Letters in Applied Microbiology. 2006 Mar;42(3):284-288. Epub 2006 Jan 25. doi: 10.1111/j.1472-765X.2005.01831.x

La, T. ; Collins, A. M. ; Phillips, N. D. et al. / Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine faeces. In: Letters in Applied Microbiology. 2006 ; Vol. 42, No. 3. pp. 284-288.