Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2

Luis G. Giménez-Lirola; Priscilla F. Gerber; Raymond R. Rowland; Patrick G. Halbur; Yao-Wei Huang; Xiang-Jin Meng; Tanja Opriessnig

doi:10.1016/j.rvsc.2014.02.014

Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2

Luis G. Giménez-Lirola, Priscilla F. Gerber, Raymond R. Rowland, Patrick G. Halbur, Yao-Wei Huang, Xiang-Jin Meng, Tanja Opriessnig

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

6 Citations (Scopus)

Abstract

A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem. © 2014.

Original language	English
Pages (from-to)	543-550
Journal	Research in Veterinary Science
Volume	96
Issue number	3
DOIs	https://doi.org/10.1016/j.rvsc.2014.02.014
Publication status	Published - Jun 2014
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]

Research Keywords

4-plex
Antibody detection
Fluorescent microbead-based immunoassay
Porcine reproductive and respiratory syndrome virus
PRRSV
Torque teno sus virus
TTSuV

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Giménez-Lirola, L. G., Gerber, P. F., Rowland, R. R., Halbur, P. G., Huang, Y.-W., Meng, X.-J., & Opriessnig, T. (2014). Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2. Research in Veterinary Science, 96(3), 543-550. https://doi.org/10.1016/j.rvsc.2014.02.014

Giménez-Lirola, Luis G. ; Gerber, Priscilla F. ; Rowland, Raymond R. et al. / Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2. In: Research in Veterinary Science. 2014 ; Vol. 96, No. 3. pp. 543-550.

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title = "Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2",

abstract = "A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem. {\textcopyright} 2014.",

keywords = "4-plex, Antibody detection, Fluorescent microbead-based immunoassay, Porcine reproductive and respiratory syndrome virus, PRRSV, Torque teno sus virus, TTSuV",

author = "Gim{\'e}nez-Lirola, {Luis G.} and Gerber, {Priscilla F.} and Rowland, {Raymond R.} and Halbur, {Patrick G.} and Yao-Wei Huang and Xiang-Jin Meng and Tanja Opriessnig",

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language = "English",

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Giménez-Lirola, LG, Gerber, PF, Rowland, RR, Halbur, PG, Huang, Y-W, Meng, X-J & Opriessnig, T 2014, 'Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2', Research in Veterinary Science, vol. 96, no. 3, pp. 543-550. https://doi.org/10.1016/j.rvsc.2014.02.014

Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2. / Giménez-Lirola, Luis G.; Gerber, Priscilla F.; Rowland, Raymond R. et al.
In: Research in Veterinary Science, Vol. 96, No. 3, 06.2014, p. 543-550.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2

AU - Giménez-Lirola, Luis G.

AU - Gerber, Priscilla F.

AU - Rowland, Raymond R.

AU - Halbur, Patrick G.

AU - Huang, Yao-Wei

AU - Meng, Xiang-Jin

AU - Opriessnig, Tanja

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]

PY - 2014/6

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KW - 4-plex

KW - Antibody detection

KW - Fluorescent microbead-based immunoassay

KW - Porcine reproductive and respiratory syndrome virus

KW - PRRSV

KW - Torque teno sus virus

KW - TTSuV

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DO - 10.1016/j.rvsc.2014.02.014

M3 - RGC 21 - Publication in refereed journal

C2 - 24650623

SN - 0034-5288

VL - 96

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EP - 550

JO - Research in Veterinary Science

JF - Research in Veterinary Science

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ER -

Giménez-Lirola LG, Gerber PF, Rowland RR, Halbur PG, Huang YW, Meng XJ et al. Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2. Research in Veterinary Science. 2014 Jun;96(3):543-550. doi: 10.1016/j.rvsc.2014.02.014

Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2

Abstract

Bibliographical note

Research Keywords

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