Development and evaluation of pH-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 2889-2898 |
Journal / Publication | International Journal of Nanomedicine |
Volume | 6 |
Online published | 16 Nov 2011 |
Publication status | Published - 2011 |
Link(s)
DOI | DOI |
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Attachment(s) | Documents
Publisher's Copyright Statement
|
Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-84859134575&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(9fecf574-27c8-4ab1-aea6-ee7412775551).html |
Abstract
Single-walled carbon nanotubes (SWNTs) have been identified as an efficient drug
carrier. Here a controlled drug-delivery system based on SWNTs coated with doxorubicin
(DOX) through hydrazone bonds was developed, because the hydrazone bond is more sensitive
to tumor microenvironments than other covalent linkers. The SWNTs were firstly stabilized with
polyethylene glycol (H2
N-PEG-NH2
). Hydrazinobenzoic acid (HBA) was then covalently attached
on SWNTs via carbodiimide-activated coupling reaction to form hydrazine-modified SWNTs.
The anticancer drug DOX was conjugated to the HBA segments of SWNT using hydrazine as
the linker. The resulting hydrazone bonds formed between the DOX molecules and the HBA
segments of SWNTs are acid cleavable, thereby providing a strong pH-responsive drug release,
which may facilitate effective DOX release near the acidic tumor microenvironment and thus
reduce its overall systemic toxicity. The DOX-loaded SWNTs were efficiently taken up by HepG2
tumor cells, and DOX was released intracellularly, as revealed by MTT assay and confocal
microscope observations. Compared with SWNT-DOX conjugate formed by supramolecular
interaction, the SWNT-HBA-DOX featured high weight loading and prolonged release of DOX,
and thus improved its cytotoxicity against cancer cells. This study suggests that while SWNTs
have great potential as a drug carrier, the efficient formulation strategy requires further study.
Research Area(s)
- carbon nanotubes, drug delivery, controlled release, SWNTs
Citation Format(s)
Development and evaluation of pH-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells. / Gu, Yan-Juan; Cheng, Jinping; Jin, Jiefu et al.
In: International Journal of Nanomedicine, Vol. 6, 2011, p. 2889-2898.
In: International Journal of Nanomedicine, Vol. 6, 2011, p. 2889-2898.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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