Determination of hydroxy metabolites of polycyclic aromatic hydrocarbons by fully automated solid-phase microextraction derivatization and gas chromatography-mass spectrometry

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

43 Scopus Citations
View graph of relations

Author(s)

  • Tiangang Luan
  • Shenghong Fang
  • Yin Zhong
  • Li Lin
  • Sidney M.N. Chan
  • And 2 others
  • Chongyu Lan
  • Nora F.Y. Tam

Detail(s)

Original languageEnglish
Pages (from-to)37-43
Journal / PublicationJournal of Chromatography A
Volume1173
Issue number1-2
Publication statusPublished - 30 Nov 2007

Abstract

A fully automated sample pretreatment method was developed for the detection of mono and dihydroxy metabolites of polycyclic aromatic hydrocarbons (PAHs) by gas chromatography-mass spectrometry in the selected ion monitoring mode. Direct immersion solid-phase microextraction for the extraction of target compounds and the headspace on-fiber silylation with N,O-bis(trimethylsilyl)trifluoroacetamide were performed automatically by a multipurpose autosampler (MPS2). The operating conditions including extraction time, derivatization time, ionic strength, pH, and incubation temperature were optimized. Calibration responses of nine metabolites of PAHs over a concentration range of 0.1-100 μg L-1 with a correlation coefficient of 0.999 were obtained. The detection limits of the nine metabolites in mini pore water, minimal salts medium and soil extract culture medium were in the range of 0.001-0.013, 0.002-0.024 and 0.002-0.134 μg L-1, respectively, while the respective quantification limits were 0.003-0.044, 0.005-0.081 and 0.008-0.447 μg L-1. The reliability was confirmed by the traditional solid-phase extraction method. The proposed method could be used to analyze the metabolites of PAHs degraded by microorganisms such as algae and to determine the biodegradation pathways of PAHs. © 2007 Elsevier B.V. All rights reserved.

Research Area(s)

  • Biodegradation, Metabolites, Microorganisms, Polycyclic aromatic hydrocarbons, Solid-phase microextraction (SPME)

Citation Format(s)

Determination of hydroxy metabolites of polycyclic aromatic hydrocarbons by fully automated solid-phase microextraction derivatization and gas chromatography-mass spectrometry. / Luan, Tiangang; Fang, Shenghong; Zhong, Yin; Lin, Li; Chan, Sidney M.N.; Lan, Chongyu; Tam, Nora F.Y.

In: Journal of Chromatography A, Vol. 1173, No. 1-2, 30.11.2007, p. 37-43.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review