Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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  • H. Y. Cheung
  • C. S. Cheung
  • C. K. Kong


Original languageEnglish
Pages (from-to)171-176
Journal / PublicationJournal of Chromatography A
Issue number1-2
Publication statusPublished - 28 Sep 2001


The present paper describes the development of a micellar electrokinetic chromatographic (MEKC) method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in ethanol extracts of Andrographis paniculata. Separations were carried out in a fused-silica capillary tube with UV detection at 214 nm. Good separation was achieved using a 20 mM borate buffer, containing 20 mM sodium dodecyl sulphate and 10 mM sodium cholate, adjusted to pH 8.3 at an operating voltage of 25 kV, temperature of 35°C and a hydrodynamic injection of 5 s. The method was validated with good correlation coefficients obtained (0.9986-0.9989) while relative standard deviation (RSD) of migration time was between 1.14 and 2.42. It is concluded that this method could be used for speedy and accurate qualitative and quantitative analysis of bioactive diterpenoids in andrographis herb and its derived products. © 2001 Elsevier Science B.V. All rights reserved.

Research Area(s)

  • Andrographis paniculata, Andrographolide, Deoxyandrographolide, Neoandrographolide