Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography

H. Y. Cheung; C. S. Cheung; C. K. Kong

doi:10.1016/S0021-9673(01)01160-8

Author(s)

H. Y. Cheung
C. S. Cheung
C. K. Kong

Detail(s)

Original language	English
Pages (from-to)	171-176
Journal / Publication	Journal of Chromatography A
Volume	930
Issue number	1-2
Publication status	Published - 28 Sept 2001

Link(s)

DOI	DOI https://doi.org/10.1016/S0021-9673(01)01160-8 Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-0035964762&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(30c6f751-4e82-414d-81b7-bd12d6c985e6).html

Abstract

The present paper describes the development of a micellar electrokinetic chromatographic (MEKC) method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in ethanol extracts of Andrographis paniculata. Separations were carried out in a fused-silica capillary tube with UV detection at 214 nm. Good separation was achieved using a 20 mM borate buffer, containing 20 mM sodium dodecyl sulphate and 10 mM sodium cholate, adjusted to pH 8.3 at an operating voltage of 25 kV, temperature of 35°C and a hydrodynamic injection of 5 s. The method was validated with good correlation coefficients obtained (0.9986-0.9989) while relative standard deviation (RSD) of migration time was between 1.14 and 2.42. It is concluded that this method could be used for speedy and accurate qualitative and quantitative analysis of bioactive diterpenoids in andrographis herb and its derived products. © 2001 Elsevier Science B.V. All rights reserved.

Research Area(s)

Andrographis paniculata, Andrographolide, Deoxyandrographolide, Neoandrographolide

Citation Format(s)

[ RIS ] [ BibTeX ]

Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography. / Cheung, H. Y.; Cheung, C. S.; Kong, C. K.
In: Journal of Chromatography A, Vol. 930, No. 1-2, 28.09.2001, p. 171-176.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Cheung, HY, Cheung, CS & Kong, CK 2001, 'Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography', Journal of Chromatography A, vol. 930, no. 1-2, pp. 171-176. https://doi.org/10.1016/S0021-9673(01)01160-8

Cheung, H. Y., Cheung, C. S., & Kong, C. K. (2001). Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography. Journal of Chromatography A, 930(1-2), 171-176. https://doi.org/10.1016/S0021-9673(01)01160-8

Cheung HY, Cheung CS, Kong CK. Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography. Journal of Chromatography A. 2001 Sept 28;930(1-2):171-176. doi: 10.1016/S0021-9673(01)01160-8

Cheung, H. Y. ; Cheung, C. S. ; Kong, C. K. / Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography. In: Journal of Chromatography A. 2001 ; Vol. 930, No. 1-2. pp. 171-176.