TY - JOUR
T1 - Determination of bioactive diterpenoids from Andrographis paniculata by micellar electrokinetic chromatography
AU - Cheung, H. Y.
AU - Cheung, C. S.
AU - Kong, C. K.
PY - 2001/9/28
Y1 - 2001/9/28
N2 - The present paper describes the development of a micellar electrokinetic chromatographic (MEKC) method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in ethanol extracts of Andrographis paniculata. Separations were carried out in a fused-silica capillary tube with UV detection at 214 nm. Good separation was achieved using a 20 mM borate buffer, containing 20 mM sodium dodecyl sulphate and 10 mM sodium cholate, adjusted to pH 8.3 at an operating voltage of 25 kV, temperature of 35°C and a hydrodynamic injection of 5 s. The method was validated with good correlation coefficients obtained (0.9986-0.9989) while relative standard deviation (RSD) of migration time was between 1.14 and 2.42. It is concluded that this method could be used for speedy and accurate qualitative and quantitative analysis of bioactive diterpenoids in andrographis herb and its derived products. © 2001 Elsevier Science B.V. All rights reserved.
AB - The present paper describes the development of a micellar electrokinetic chromatographic (MEKC) method for simultaneous determination of andrographolide, deoxyandrographolide and neoandrographolide in ethanol extracts of Andrographis paniculata. Separations were carried out in a fused-silica capillary tube with UV detection at 214 nm. Good separation was achieved using a 20 mM borate buffer, containing 20 mM sodium dodecyl sulphate and 10 mM sodium cholate, adjusted to pH 8.3 at an operating voltage of 25 kV, temperature of 35°C and a hydrodynamic injection of 5 s. The method was validated with good correlation coefficients obtained (0.9986-0.9989) while relative standard deviation (RSD) of migration time was between 1.14 and 2.42. It is concluded that this method could be used for speedy and accurate qualitative and quantitative analysis of bioactive diterpenoids in andrographis herb and its derived products. © 2001 Elsevier Science B.V. All rights reserved.
KW - Andrographis paniculata
KW - Andrographolide
KW - Deoxyandrographolide
KW - Neoandrographolide
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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-0035964762&origin=recordpage
U2 - 10.1016/S0021-9673(01)01160-8
DO - 10.1016/S0021-9673(01)01160-8
M3 - RGC 21 - Publication in refereed journal
C2 - 11681575
SN - 0021-9673
VL - 930
SP - 171
EP - 176
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -