Determination of Antioxidants in Smilacis Glabrae Rhizoma by High-Performance Liquid Chromatography with Ultraviolet and Mass Spectrometry Detection

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

2 Scopus Citations
View graph of relations

Author(s)

  • Zhi-Feng Zhang
  • Lu-Yang Lu
  • Yuan Liu
  • Rui Zeng
  • Jing Xie
  • And 3 others
  • Ye-Qing Huang
  • Qing Shen
  • Hon-Yeung Cheung

Detail(s)

Original languageEnglish
Pages (from-to)1975-1985
Journal / PublicationAnalytical Letters
Volume49
Issue number13
Publication statusPublished - 1 Sep 2016

Abstract

ABSTRACT: Smilacis Glabrae Rhizoma is a traditional food in China. Although phytochemical and pharmacology studies have been performed, the antioxidant constituents have not been characterized with the exception of astilbin. In the present study, six antioxidants in Smilacis Glabrae Rhizoma were determined by high-performance liquid chromatography with ultraviolet and mass spectrometry detection using 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) assays. These analytes were 5-O-caffeoylshikimic acid, polydatin, neoastilbin, astilbin, neoisoastilbin, and isoastilbin. The relative Trolox equivalent antioxidant capacity of each compound was determined using the two protocols. Moreover, antioxidant fingerprints were constructed for quality evaluation of Smilacis Glabrae Rhizoma and principal component analysis was performed to provide a guide for customers and producers to ensure the quality of the raw materials. The methodology may be used for the analysis of Smilacis Glabrae Rhizoma and potentially for other plant-based products.

Research Area(s)

  • Antioxidant activity, electrospray ionization mass spectrometry, high-performance liquid chromatography, radical scavengers, Smilacis Glabrae Rhizoma

Citation Format(s)

Determination of Antioxidants in Smilacis Glabrae Rhizoma by High-Performance Liquid Chromatography with Ultraviolet and Mass Spectrometry Detection. / Zhang, Zhi-Feng; Lu, Lu-Yang; Liu, Yuan; Zeng, Rui; Xie, Jing; Huang, Ye-Qing; Shen, Qing; Cheung, Hon-Yeung.

In: Analytical Letters, Vol. 49, No. 13, 01.09.2016, p. 1975-1985.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review