Determination of Adenosine Triphosphate by a Target Inhibited Catalytic Cycle Based on a Strand Displacement Reaction

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Sheng Cheng
  • Bin Zheng
  • Mozhen Wang
  • Qing Zhao
  • Michael Hon-Wah Lam
  • And 1 others
  • Xuewu Ge

Detail(s)

Original languageEnglish
Pages (from-to)478-491
Journal / PublicationAnalytical Letters
Volume47
Issue number3
Online published5 Feb 2014
Publication statusPublished - 2014

Abstract

A strand displacement reaction-based system was developed for the determination of adenosine triphosphate (ATP). It involved an entropy-driven catalytic cycle that directly employed the ATP aptamer as the catalyst. Introduction of ATP into the system induced the catalyst to form the G-quadruplex conformation and inhibited its catalytic activity. All intermediates in the catalytic cycle processes were identified by polyacrylamide gel electrophoresis analysis. When the oligonucleotides were labeled with a carboxyfluorescein fluorophore and a 4-([4-(dimethylamino)phenyl]azo)benzoic acid quencher, this strand displacement reaction-based catalytic system exhibited a "switch-on" response for ATP. Conditions for detecting ATP, such as the toehold length, concentrations of the catalyst and magnesium ion, and incubation temperature, were optimized to obtain a detection limit of 50 nM and a linear response up to 1400 nM of ATP. This target inhibited catalytic cycle provides an enzyme-free biosensing strategy and has potential application in aptamer-based biosensing. © 2014 Copyright Taylor and Francis Group, LLC.

Research Area(s)

  • Adenosine triphosphate, Amplification detection, Aptamer, Enzyme-free, Strand displacement reaction

Citation Format(s)

Determination of Adenosine Triphosphate by a Target Inhibited Catalytic Cycle Based on a Strand Displacement Reaction. / Cheng, Sheng; Zheng, Bin; Wang, Mozhen; Zhao, Qing; Lam, Michael Hon-Wah; Ge, Xuewu.

In: Analytical Letters, Vol. 47, No. 3, 2014, p. 478-491.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review