Detection of multiple network-based allosteric interactions between peptides and arrays of DNA binding sites

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

6 Scopus Citations
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Author(s)

  • Karen K.L. Kao
  • Jonathan C.T. Huang
  • Chi-Kai Yang
  • Kee-Ching G. Jeng
  • Jung-Cheng Chang
  • Wen-Chen Yao
  • S. C. Hsien
  • Michael J. Waring
  • Lin Ma
  • Leung Sheh

Detail(s)

Original languageEnglish
Pages (from-to)366-376
Journal / PublicationBioorganic and Medicinal Chemistry
Volume18
Issue number1
Online published31 Oct 2009
Publication statusPublished - 1 Jan 2010
Externally publishedYes

Abstract

Quantitative DNase I footprinting shows that three designed peptides containing N-methylpyrrole (Py) moieties display different types of network-based allosteric communication in binding to DNA: circuit type, incomplete-circuit type, and non-circuit type characterized by interstrand bidentate interactions. Positive cooperative binding of all three peptides to individual DNA binding sites is commonly observed. CD spectral characterization of the interaction between peptides and model undecanucleotide duplexes is consistent with the footprinting results and supports the allosteric model. This study provides insights relating to the interaction network nature of allostery in complex DNA-small molecule interactions. © 2009 Elsevier Ltd. All rights reserved.

Research Area(s)

  • Circular dichroism, DNA cooperative networks, Footprinting, Molecular recognition, Peptides

Citation Format(s)

Detection of multiple network-based allosteric interactions between peptides and arrays of DNA binding sites. / Kao, Karen K.L.; Huang, Jonathan C.T.; Yang, Chi-Kai; Jeng, Kee-Ching G.; Chang, Jung-Cheng; Yao, Wen-Chen; Hsien, S. C.; Waring, Michael J.; Chen, Ming-Hua; Ma, Lin; Sheh, Leung.

In: Bioorganic and Medicinal Chemistry, Vol. 18, No. 1, 01.01.2010, p. 366-376.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal