DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM

Hong-Lin Cheng; Chien-Yu Fu; Yen-Wen Chen; Yu-Lin Wang; Chih-Chen Chen; Gwo-Bin Lee

doi:10.1109/MEMSYS.2018.8346779

DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM

Hong-Lin Cheng
, Chien-Yu Fu
, Yen-Wen Chen
, Yu-Lin Wang
, Chih-Chen Chen
, Gwo-Bin Lee^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Recently, micro ribonucleic acids (miRNAs) extracted from extracellular vesicles (EVs) have been recognized as promising biomarkers for early detection of cardiovascular diseases (CVDs). However, the detection and quantification of miRNAs by using the existing methods are relatively labor-intensive and time-consuming. In this work, an integrated microfluidic system equipped with highly sensitive field-effect transistors which is capable of performing EVs extraction, EVs lysis, target miRNAs extraction and miRNAs detection was reported. The entire detection process could be automated within three hours on a single chip and the detection limit of miRNAs was observed to be in a femtomolar range, which meets the requirement of physiological concentrations. This integrated microfluidic system has great potential to be a tool for early detection of CVDs.

Original language	English
Title of host publication	2018 IEEE Micro Electro Mechanical Systems (MEMS)
Publisher	IEEE
Pages	1205-1208
ISBN (Electronic)	9781538647820
DOIs	https://doi.org/10.1109/MEMSYS.2018.8346779
Publication status	Published - Jan 2018
Externally published	Yes
Event	31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018) - Belfast, United Kingdom Duration: 21 Jan 2018 → 25 Jan 2018 https://www.mems2018.org/

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Publisher	IEEE
ISSN (Print)	1084-6999
ISSN (Electronic)	2160-1968

Conference

Conference	31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018)
Place	United Kingdom
City	Belfast
Period	21/01/18 → 25/01/18
Internet address	https://www.mems2018.org/

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1109/MEMSYS.2018.8346779

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Cheng, H.-L., Fu, C.-Y., Chen, Y.-W., Wang, Y.-L., Chen, C.-C., & Lee, G.-B. (2018). DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM. In 2018 IEEE Micro Electro Mechanical Systems (MEMS) (pp. 1205-1208). (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). IEEE. https://doi.org/10.1109/MEMSYS.2018.8346779

Cheng, Hong-Lin ; Fu, Chien-Yu ; Chen, Yen-Wen et al. / DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM. 2018 IEEE Micro Electro Mechanical Systems (MEMS) . IEEE, 2018. pp. 1205-1208 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

@inproceedings{56f59177dbc34b3aa539f31cc8770dc9,

title = "DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM",

abstract = "Recently, micro ribonucleic acids (miRNAs) extracted from extracellular vesicles (EVs) have been recognized as promising biomarkers for early detection of cardiovascular diseases (CVDs). However, the detection and quantification of miRNAs by using the existing methods are relatively labor-intensive and time-consuming. In this work, an integrated microfluidic system equipped with highly sensitive field-effect transistors which is capable of performing EVs extraction, EVs lysis, target miRNAs extraction and miRNAs detection was reported. The entire detection process could be automated within three hours on a single chip and the detection limit of miRNAs was observed to be in a femtomolar range, which meets the requirement of physiological concentrations. This integrated microfluidic system has great potential to be a tool for early detection of CVDs.",

author = "Hong-Lin Cheng and Chien-Yu Fu and Yen-Wen Chen and Yu-Lin Wang and Chih-Chen Chen and Gwo-Bin Lee",

year = "2018",

month = jan,

doi = "10.1109/MEMSYS.2018.8346779",

language = "English",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "IEEE",

pages = "1205--1208",

booktitle = "2018 IEEE Micro Electro Mechanical Systems (MEMS)",

address = "United States",

note = "31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018) ; Conference date: 21-01-2018 Through 25-01-2018",

url = "https://www.mems2018.org/",

}

Cheng, H-L, Fu, C-Y, Chen, Y-W, Wang, Y-L, Chen, C-C & Lee, G-B 2018, DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM. in 2018 IEEE Micro Electro Mechanical Systems (MEMS) . Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), IEEE, pp. 1205-1208, 31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018), Belfast, United Kingdom, 21/01/18. https://doi.org/10.1109/MEMSYS.2018.8346779

DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM. / Cheng, Hong-Lin; Fu, Chien-Yu; Chen, Yen-Wen et al.
2018 IEEE Micro Electro Mechanical Systems (MEMS) . IEEE, 2018. p. 1205-1208 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM

AU - Cheng, Hong-Lin

AU - Fu, Chien-Yu

AU - Chen, Yen-Wen

AU - Wang, Yu-Lin

AU - Chen, Chih-Chen

AU - Lee, Gwo-Bin

PY - 2018/1

Y1 - 2018/1

N2 - Recently, micro ribonucleic acids (miRNAs) extracted from extracellular vesicles (EVs) have been recognized as promising biomarkers for early detection of cardiovascular diseases (CVDs). However, the detection and quantification of miRNAs by using the existing methods are relatively labor-intensive and time-consuming. In this work, an integrated microfluidic system equipped with highly sensitive field-effect transistors which is capable of performing EVs extraction, EVs lysis, target miRNAs extraction and miRNAs detection was reported. The entire detection process could be automated within three hours on a single chip and the detection limit of miRNAs was observed to be in a femtomolar range, which meets the requirement of physiological concentrations. This integrated microfluidic system has great potential to be a tool for early detection of CVDs.

AB - Recently, micro ribonucleic acids (miRNAs) extracted from extracellular vesicles (EVs) have been recognized as promising biomarkers for early detection of cardiovascular diseases (CVDs). However, the detection and quantification of miRNAs by using the existing methods are relatively labor-intensive and time-consuming. In this work, an integrated microfluidic system equipped with highly sensitive field-effect transistors which is capable of performing EVs extraction, EVs lysis, target miRNAs extraction and miRNAs detection was reported. The entire detection process could be automated within three hours on a single chip and the detection limit of miRNAs was observed to be in a femtomolar range, which meets the requirement of physiological concentrations. This integrated microfluidic system has great potential to be a tool for early detection of CVDs.

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DO - 10.1109/MEMSYS.2018.8346779

M3 - RGC 32 - Refereed conference paper (with host publication)

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 1205

EP - 1208

BT - 2018 IEEE Micro Electro Mechanical Systems (MEMS)

PB - IEEE

T2 - 31st IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2018)

Y2 - 21 January 2018 through 25 January 2018

ER -

Cheng HL, Fu CY, Chen YW, Wang YL, Chen CC, Lee GB. DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM. In 2018 IEEE Micro Electro Mechanical Systems (MEMS) . IEEE. 2018. p. 1205-1208. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2018.8346779

DETECTION OF MICRO RIBONUCLEIC ACIDS FROM EXTRACTED EXTRACELLULAR VESICLES FOR CARDIOVASCULAR DISEASES BY USING AN INTEGRATED MICROFLUIDIC SYSTEM

Abstract

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Conference

UN SDGs

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