Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily

K. Georges; G. R. Loria; S. Riili; A. Greco; S. Caracappa; F. Jongejan; O. Sparagano

doi:10.1016/S0304-4017(01)00488-5

Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily

K. Georges
, G. R. Loria
, S. Riili
, A. Greco
, S. Caracappa
, F. Jongejan
, O. Sparagano

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

230 Citations (Scopus)

Abstract

A reverse line blot hybridisation (RLB) of 21 oligonucleotides with polymerase chain reaction (PCR) amplified regions of 16S rRNA (Ehrlichia/Anaplasma group) or 18S rRNA (Babesia/Theileria group) genes of haemoparasites detected Theileria annulata, T. buffeli/orientalis, Babesia bovis, B. bigemina, B. divergens, Ehrlichia bovis, Anaplasma marginale, A. centrale and unknown species within the Rickettsia tribe.
A very high prevalence of mixed infections was detected, which indicated that animals infected with Babesia spp. were also infected with Theileria spp. and/or Anaplasma spp.
The tick distribution appeared to be seasonal with Hyalomma marginatum as the most frequently observed tick and Boophilus annulatus and Ixodes ricinus as the least frequently observed ticks. Other species identified in the 818 ticks collected during the five sampling periods between April 1998 and November 1999 included H. lusitanicum, Rhipicephalus sanguineus group, R. bursa, Dermacentor marginatus, Haemaphysalis punctata, B. annulatus and I. ricinus.

Original language	English
Pages (from-to)	273-286
Journal	Veterinary Parasitology
Volume	99
Issue number	4
Online published	13 Aug 2001
DOIs	https://doi.org/10.1016/S0304-4017(01)00488-5
Publication status	Published - 31 Aug 2001
Externally published	Yes

Research Keywords

Cattle
Epidemiology
PCR
Reverse line blot
Sicily
Tick-borne diseases
Ticks

Policy Impact

Cited in Policy Documents

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10.1016/S0304-4017(01)00488-5

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title = "Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily",

abstract = " A reverse line blot hybridisation (RLB) of 21 oligonucleotides with polymerase chain reaction (PCR) amplified regions of 16S rRNA (Ehrlichia/Anaplasma group) or 18S rRNA (Babesia/Theileria group) genes of haemoparasites detected Theileria annulata, T. buffeli/orientalis, Babesia bovis, B. bigemina, B. divergens, Ehrlichia bovis, Anaplasma marginale, A. centrale and unknown species within the Rickettsia tribe. A very high prevalence of mixed infections was detected, which indicated that animals infected with Babesia spp. were also infected with Theileria spp. and/or Anaplasma spp. The tick distribution appeared to be seasonal with Hyalomma marginatum as the most frequently observed tick and Boophilus annulatus and Ixodes ricinus as the least frequently observed ticks. Other species identified in the 818 ticks collected during the five sampling periods between April 1998 and November 1999 included H. lusitanicum, Rhipicephalus sanguineus group, R. bursa, Dermacentor marginatus, Haemaphysalis punctata, B. annulatus and I. ricinus. ",

keywords = "Cattle, Epidemiology, PCR, Reverse line blot, Sicily, Tick-borne diseases, Ticks",

author = "K. Georges and Loria, \{G. R.\} and S. Riili and A. Greco and S. Caracappa and F. Jongejan and O. Sparagano",

year = "2001",

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doi = "10.1016/S0304-4017(01)00488-5",

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pages = "273--286",

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TY - JOUR

T1 - Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily

AU - Georges, K.

AU - Loria, G. R.

AU - Riili, S.

AU - Greco, A.

AU - Caracappa, S.

AU - Jongejan, F.

AU - Sparagano, O.

PY - 2001/8/31

Y1 - 2001/8/31

N2 - A reverse line blot hybridisation (RLB) of 21 oligonucleotides with polymerase chain reaction (PCR) amplified regions of 16S rRNA (Ehrlichia/Anaplasma group) or 18S rRNA (Babesia/Theileria group) genes of haemoparasites detected Theileria annulata, T. buffeli/orientalis, Babesia bovis, B. bigemina, B. divergens, Ehrlichia bovis, Anaplasma marginale, A. centrale and unknown species within the Rickettsia tribe. A very high prevalence of mixed infections was detected, which indicated that animals infected with Babesia spp. were also infected with Theileria spp. and/or Anaplasma spp. The tick distribution appeared to be seasonal with Hyalomma marginatum as the most frequently observed tick and Boophilus annulatus and Ixodes ricinus as the least frequently observed ticks. Other species identified in the 818 ticks collected during the five sampling periods between April 1998 and November 1999 included H. lusitanicum, Rhipicephalus sanguineus group, R. bursa, Dermacentor marginatus, Haemaphysalis punctata, B. annulatus and I. ricinus.

AB - A reverse line blot hybridisation (RLB) of 21 oligonucleotides with polymerase chain reaction (PCR) amplified regions of 16S rRNA (Ehrlichia/Anaplasma group) or 18S rRNA (Babesia/Theileria group) genes of haemoparasites detected Theileria annulata, T. buffeli/orientalis, Babesia bovis, B. bigemina, B. divergens, Ehrlichia bovis, Anaplasma marginale, A. centrale and unknown species within the Rickettsia tribe. A very high prevalence of mixed infections was detected, which indicated that animals infected with Babesia spp. were also infected with Theileria spp. and/or Anaplasma spp. The tick distribution appeared to be seasonal with Hyalomma marginatum as the most frequently observed tick and Boophilus annulatus and Ixodes ricinus as the least frequently observed ticks. Other species identified in the 818 ticks collected during the five sampling periods between April 1998 and November 1999 included H. lusitanicum, Rhipicephalus sanguineus group, R. bursa, Dermacentor marginatus, Haemaphysalis punctata, B. annulatus and I. ricinus.

KW - Cattle

KW - Epidemiology

KW - PCR

KW - Reverse line blot

KW - Sicily

KW - Tick-borne diseases

KW - Ticks

UR - https://www.scopus.com/pages/publications/0035979825

UR - https://www.scopus.com/pages/publications/0035979825#tab=impact

UR - https://app-overton-io.ezproxy.cityu.edu.hk/articles.php?query=10.1016/S0304-4017(01)00488-5

U2 - 10.1016/S0304-4017(01)00488-5

DO - 10.1016/S0304-4017(01)00488-5

M3 - RGC 21 - Publication in refereed journal

C2 - 11511414

SN - 0304-4017

VL - 99

SP - 273

EP - 286

JO - Veterinary Parasitology

JF - Veterinary Parasitology

IS - 4

ER -

Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily

Abstract

Research Keywords

Policy Impact

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