Detection of AT1R on H295R cells by functionalized AFM tip
Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45) › 32_Refereed conference paper (with ISBN/ISSN) › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
| Original language | English |
|---|---|
| Title of host publication | IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED |
| Publisher | IEEE |
| Pages | 57-60 |
| ISBN (Print) | 9781509046751 |
| Publication status | Published - 20 Mar 2017 |
Publication series
| Name | |
|---|---|
| ISSN (Print) | 2159-6964 |
| ISSN (Electronic) | 2159-6972 |
Conference
| Title | 10th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2016 |
|---|---|
| Place | China |
| City | Macau |
| Period | 30 October - 2 November 2016 |
Link(s)
| DOI | DOI |
|---|---|
| Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(1509477b-db4c-4bff-83e0-93aa3e91206d).html |
Abstract
Specific molecular recognition between antibody and receptor on cell membrane plays a central role in biological research. As a main component in the renin-Angiotensin system (RAS), angiotensin II type I receptor (AT1R) mediates the classical biological actions of angiotensin II (Ang II). In this study, we applied atomic force microscopy (AFM) to characterize and quantify binding interaction between anti-AT1R antibody and AT1R on H295R cells. Immunocytochemistry analysis of AT1R was performed on H295R cells to confirm the expression and localization of AT1R. AFM measurement showed the specific unbinding force between anti-AT1R antibody and AT1R was in the range of 50-120 pN. This investigation provides new avenues for mapping AT1R on living cells.
Citation Format(s)
Detection of AT1R on H295R cells by functionalized AFM tip. / Gao, Qi; Chiu Lai, King Wai.
IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. IEEE, 2017. p. 57-60 7883487.Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45) › 32_Refereed conference paper (with ISBN/ISSN) › peer-review
