Detection of AT1R on H295R cells by functionalized AFM tip

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)

View graph of relations

Detail(s)

Original languageEnglish
Title of host publicationIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
PublisherIEEE
Pages57-60
ISBN (Print)9781509046751
Publication statusPublished - 20 Mar 2017

Publication series

Name
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Conference

Title10th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2016
PlaceChina
CityMacau
Period30 October - 2 November 2016

Abstract

Specific molecular recognition between antibody and receptor on cell membrane plays a central role in biological research. As a main component in the renin-Angiotensin system (RAS), angiotensin II type I receptor (AT1R) mediates the classical biological actions of angiotensin II (Ang II). In this study, we applied atomic force microscopy (AFM) to characterize and quantify binding interaction between anti-AT1R antibody and AT1R on H295R cells. Immunocytochemistry analysis of AT1R was performed on H295R cells to confirm the expression and localization of AT1R. AFM measurement showed the specific unbinding force between anti-AT1R antibody and AT1R was in the range of 50-120 pN. This investigation provides new avenues for mapping AT1R on living cells.

Citation Format(s)

Detection of AT1R on H295R cells by functionalized AFM tip. / Gao, Qi; Chiu Lai, King Wai.

IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. IEEE, 2017. p. 57-60 7883487.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)