Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Yuzhao Zhang
  • Junhua Zhao
  • Haibo Yu
  • Pan Li
  • Wenfeng Liang
  • Zhu Liu
  • Gwo-Bin Lee
  • Lianqing Liu
  • Zhenning Wang

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article numbereaba9628
Journal / PublicationScience Advances
Volume6
Issue number32
Online published5 Aug 2020
Publication statusPublished - 2020

Link(s)

Abstract

Detection of free gastric cancer cells in peritoneal lavages and ascites plays a vital role in gastric cancer. However, due to the low content of cancer cells in patients’ peritoneal lavages, traditional detection methods lack sensitivity and cannot satisfy clinical demand. In this study, we used an optically induced electrokinetics (OEK) microfluidic method for label-free separation and characterization of patient gastric cancer cells. This method showed high effectiveness and sensitivity. We successfully separated cancer cells from a simulated peritoneal lavage mixture of gastric cancer cell lines and peritoneal lavage cells in a ratio of 1:1000. We further separated gastric cancer cells from six patients’ ascites with purity up to 71%. In addition, we measured the cell membrane capacitances, which may be used as a biomarker for gastric cancer cells. Thus, our method can be used to effectively and rapidly detect peritoneal metastasis and to acquire cellular electrical information.

Citation Format(s)

Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK). / Zhang, Yuzhao; Zhao, Junhua; Yu, Haibo et al.
In: Science Advances, Vol. 6, No. 32, eaba9628, 2020.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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