Design and synthesis of heterobimetallic donor-acceptor chemodosimetric ensembles for the detection of sulfhydryl-containing amino acids and peptides

Cheuk-Fai Chow; Michael H. W. Lam; Hongyan Sui; Wai-Yeung Wong

doi:10.1039/b410974k

Design and synthesis of heterobimetallic donor-acceptor chemodosimetric ensembles for the detection of sulfhydryl-containing amino acids and peptides

Cheuk-Fai Chow
, Michael H. W. Lam
, Hongyan Sui
, Wai-Yeung Wong

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

20 Citations (Scopus)

Abstract

A competitive indicator displacement assay has been successfully developed for the ratiometric determination of sulfhydryl-containing amino acids and peptides using heterobimetallic donor-acceptor complexes as chemodosimetric ensembles. Chromotropic cis-[ML₂(CN)₂] (M = Fe^II, Ru^II, Os^II; L = diimine) are used as signaling indicators and Pt^II(DMSO)Cl₂ acceptor moiety is used as the receptor for the sulfhydryl-containing analytes. A series of three heterobimetallic donor-acceptor complexes: cis-Fe^II(bpy) ₂[CN-Pt^II(DMSO)Cl₂]₂ (1), cir-Ru^II(bpy)₂[CN-Pt^II(DMSO)Cl₂]₂ (2) and cis-Os^II(bpy)₂[CN- Pt^II(DMSO)Cl₂]₂ (3) are synthesized and characterized by X-ray crystallography. All the three ensembles are able to produce specific colorimetric/fluorimetric responses to sulfhydryl-containing amino acids (cysteine, homocysteine and methionine) as well as the sulfhydryl-containing small peptide glutathione. The mechanism of the competitive displacement assay is evaluated by examining the thermodynamics of formation of the donor-acceptor linkage and adducts between the acceptor metal and the sulfhydryl-containing analytes as well as by systematic variation of the donor and acceptor metals in the chemodosimetric ensembles.

Original language	English
Pages (from-to)	475-484
Journal	Dalton Transactions
Issue number	3
Online published	5 Jan 2005
DOIs	https://doi.org/10.1039/b410974k
Publication status	Published - 7 Feb 2005

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@article{ab78c8d3b3c541878ac915a67714f671,

title = "Design and synthesis of heterobimetallic donor-acceptor chemodosimetric ensembles for the detection of sulfhydryl-containing amino acids and peptides",

abstract = "A competitive indicator displacement assay has been successfully developed for the ratiometric determination of sulfhydryl-containing amino acids and peptides using heterobimetallic donor-acceptor complexes as chemodosimetric ensembles. Chromotropic cis-[ML2(CN)2] (M = FeII, RuII, OsII; L = diimine) are used as signaling indicators and PtII(DMSO)Cl2 acceptor moiety is used as the receptor for the sulfhydryl-containing analytes. A series of three heterobimetallic donor-acceptor complexes: cis-FeII(bpy) 2[CN-PtII(DMSO)Cl2]2 (1), cir-RuII(bpy)2[CN-PtII(DMSO)Cl2]2 (2) and cis-OsII(bpy)2[CN- PtII(DMSO)Cl2]2 (3) are synthesized and characterized by X-ray crystallography. All the three ensembles are able to produce specific colorimetric/fluorimetric responses to sulfhydryl-containing amino acids (cysteine, homocysteine and methionine) as well as the sulfhydryl-containing small peptide glutathione. The mechanism of the competitive displacement assay is evaluated by examining the thermodynamics of formation of the donor-acceptor linkage and adducts between the acceptor metal and the sulfhydryl-containing analytes as well as by systematic variation of the donor and acceptor metals in the chemodosimetric ensembles.",

author = "Cheuk-Fai Chow and Lam, \{Michael H. W.\} and Hongyan Sui and Wai-Yeung Wong",

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Design and synthesis of heterobimetallic donor-acceptor chemodosimetric ensembles for the detection of sulfhydryl-containing amino acids and peptides. / Chow, Cheuk-Fai; Lam, Michael H. W.; Sui, Hongyan et al.
In: Dalton Transactions, No. 3, 07.02.2005, p. 475-484.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Design and synthesis of heterobimetallic donor-acceptor chemodosimetric ensembles for the detection of sulfhydryl-containing amino acids and peptides

AU - Chow, Cheuk-Fai

AU - Lam, Michael H. W.

AU - Sui, Hongyan

AU - Wong, Wai-Yeung

PY - 2005/2/7

Y1 - 2005/2/7

N2 - A competitive indicator displacement assay has been successfully developed for the ratiometric determination of sulfhydryl-containing amino acids and peptides using heterobimetallic donor-acceptor complexes as chemodosimetric ensembles. Chromotropic cis-[ML2(CN)2] (M = FeII, RuII, OsII; L = diimine) are used as signaling indicators and PtII(DMSO)Cl2 acceptor moiety is used as the receptor for the sulfhydryl-containing analytes. A series of three heterobimetallic donor-acceptor complexes: cis-FeII(bpy) 2[CN-PtII(DMSO)Cl2]2 (1), cir-RuII(bpy)2[CN-PtII(DMSO)Cl2]2 (2) and cis-OsII(bpy)2[CN- PtII(DMSO)Cl2]2 (3) are synthesized and characterized by X-ray crystallography. All the three ensembles are able to produce specific colorimetric/fluorimetric responses to sulfhydryl-containing amino acids (cysteine, homocysteine and methionine) as well as the sulfhydryl-containing small peptide glutathione. The mechanism of the competitive displacement assay is evaluated by examining the thermodynamics of formation of the donor-acceptor linkage and adducts between the acceptor metal and the sulfhydryl-containing analytes as well as by systematic variation of the donor and acceptor metals in the chemodosimetric ensembles.

AB - A competitive indicator displacement assay has been successfully developed for the ratiometric determination of sulfhydryl-containing amino acids and peptides using heterobimetallic donor-acceptor complexes as chemodosimetric ensembles. Chromotropic cis-[ML2(CN)2] (M = FeII, RuII, OsII; L = diimine) are used as signaling indicators and PtII(DMSO)Cl2 acceptor moiety is used as the receptor for the sulfhydryl-containing analytes. A series of three heterobimetallic donor-acceptor complexes: cis-FeII(bpy) 2[CN-PtII(DMSO)Cl2]2 (1), cir-RuII(bpy)2[CN-PtII(DMSO)Cl2]2 (2) and cis-OsII(bpy)2[CN- PtII(DMSO)Cl2]2 (3) are synthesized and characterized by X-ray crystallography. All the three ensembles are able to produce specific colorimetric/fluorimetric responses to sulfhydryl-containing amino acids (cysteine, homocysteine and methionine) as well as the sulfhydryl-containing small peptide glutathione. The mechanism of the competitive displacement assay is evaluated by examining the thermodynamics of formation of the donor-acceptor linkage and adducts between the acceptor metal and the sulfhydryl-containing analytes as well as by systematic variation of the donor and acceptor metals in the chemodosimetric ensembles.

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M3 - RGC 21 - Publication in refereed journal

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SN - 1477-9226

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JO - Dalton Transactions

JF - Dalton Transactions

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ER -

Design and synthesis of heterobimetallic donor-acceptor chemodosimetric ensembles for the detection of sulfhydryl-containing amino acids and peptides

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