Demonstration of increased anti-mycobacterial activity in peripheral blood monocytes after BCG vaccination in British school children

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

15 Scopus Citations
View graph of relations


  • J. POOLE
  • V. R. ABER
  • K. B. WALKER
  • D. B. LOWRIE


Original languageEnglish
Pages (from-to)20-25
Journal / PublicationClinical and Experimental Immunology
Issue number1
Publication statusPublished - 1988
Externally publishedYes


A blood sample was taken from children aged 13-15 years immediately before BCG vaccination and 8 weeks later. The children were tuberculin skin-test negative to PPD-S before vaccination and positive after. Mononuclear cells were separated from the blood, infected with Mycobacterium microti at a low bacterium/monocyte ratio and allowed to form monolayers in microtitre wells. The infected monolayers were rinsed daily and the change in number of live bacteria in monolayers and supernatants was monitored by colony counts on agar. The cells were bacteriostatic during the first day, thereafter growth accelerated in pre-vaccination monolayers. When monolayers received pulsed exposures to autologous lymphocytes that had been incubated with whole dead tubercle bacilli the growth rates of M. microti were increased. However, growth rates in lymphocyte-pulsed monolayers were significantly lower after vaccination than before. It is proposed that this difference reflects the protective effect of vaccination.

Research Area(s)

  • macrophages mycobacteria, BCG vaccination

Citation Format(s)