Decrease in RNA folding cooperativity by deliberate population of intermediates in RNA G-quadruplexes

Chun Kit Kwok; Madeline E. Sherlock; Philip C. Bevilacqua

doi:10.1002/anie.201206475

Decrease in RNA folding cooperativity by deliberate population of intermediates in RNA G-quadruplexes

Chun Kit Kwok, Madeline E. Sherlock, Philip C. Bevilacqua^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

21 Citations (Scopus)

Abstract

Keeping a broad (RNA) perspective: Conventional biochemical detection systems only have a 100-fold linear response range. The range of potassium concentrations detected by an RNA G-quadruplex sequence can be broadened by intentionally populating multiple intermediate folding states (see scheme). The folding of the RNA G-quadruplexes was monitored by both circular dichroism and intrinsic fluorescence spectroscopy. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original language	English
Pages (from-to)	683-686
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	2
DOIs	https://doi.org/10.1002/anie.201206475
Publication status	Published - 7 Jan 2013
Externally published	Yes

Research Keywords

fluorescence
folding cooperativity
G-quadruplexes
RNA
RNA structures

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abstract = "Keeping a broad (RNA) perspective: Conventional biochemical detection systems only have a 100-fold linear response range. The range of potassium concentrations detected by an RNA G-quadruplex sequence can be broadened by intentionally populating multiple intermediate folding states (see scheme). The folding of the RNA G-quadruplexes was monitored by both circular dichroism and intrinsic fluorescence spectroscopy. Copyright {\textcopyright} 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

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AU - Bevilacqua, Philip C.

PY - 2013/1/7

Y1 - 2013/1/7

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AB - Keeping a broad (RNA) perspective: Conventional biochemical detection systems only have a 100-fold linear response range. The range of potassium concentrations detected by an RNA G-quadruplex sequence can be broadened by intentionally populating multiple intermediate folding states (see scheme). The folding of the RNA G-quadruplexes was monitored by both circular dichroism and intrinsic fluorescence spectroscopy. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - fluorescence

KW - folding cooperativity

KW - G-quadruplexes

KW - RNA

KW - RNA structures

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M3 - RGC 21 - Publication in refereed journal

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SP - 683

EP - 686

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

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ER -

Decrease in RNA folding cooperativity by deliberate population of intermediates in RNA G-quadruplexes

Abstract

Research Keywords

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