Cytotoxicity of three cycloartane triterpenoids from Cimicifuga dahurica

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Ze Tian
  • Feng Huang
  • Keguo Li
  • Jianyong Si
  • Lin Shi
  • Sibao Chen
  • Peigen Xiao

Detail(s)

Original languageEnglish
Pages (from-to)65-75
Journal / PublicationCancer Letters
Volume226
Issue number1
Publication statusPublished - 8 Aug 2005

Abstract

We first investigated the cytotoxicity of three cycloartane triterpenoids isolated from the aerial part of C. dahurica. Their cytotoxic activity was investigated on several cancer cell lines including solid tumor (HepG2), blood tumor (HL-60), drug resistant tumor (R-HepG2) and primary cultured normal mouse and rat hepatocytes in order to find efficient anti-tumor agents against both parental and drug-resistant tumor with reduced toxicity. Evident cytotoxicity of these compounds on all tested neoplastic cell lines revealed that they are efficient on both drug-resistant tumor and parental tumor. Furthermore, they all showed relatively selective cytotoxicity on cancerous cells based on the higher IC50 values of them on normal cells than that on tumor cells. Morphological observation and cell cycle analysis were employed to elucidate the cytotoxicity of the tested compounds. They brought out similar apoptotic morphological changes and G2/M cell cycle arrest in HepG2, R-HepG2 and HL-60 cells. Moreover, they suppressed the expression of cdc2 and COX-2 protein. These results imply that the three compounds possess potential anti-tumor activities and they exert their cytotoxicity via apoptosis and G 2/M arrest. In addition, inhibition of cdc2 protein expression correlates with mechanism of G2/M arrest. © 2004 Elsevier Ireland Ltd. All rights reserved.

Research Area(s)

  • Apoptosis, cdc 2, Cell cycle, Cycloartane triterpenoids, Cyclooxygenase-2, Cytotoxicity

Citation Format(s)

Cytotoxicity of three cycloartane triterpenoids from Cimicifuga dahurica. / Tian, Ze; Yang, Mengsu; Huang, Feng; Li, Keguo; Si, Jianyong; Shi, Lin; Chen, Sibao; Xiao, Peigen.

In: Cancer Letters, Vol. 226, No. 1, 08.08.2005, p. 65-75.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review