Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection

M. S. Chan; D. Xu; L. Guo; D. Y. Tam; L. S. Liu; Y. Chen; M. S. Wong; P. K. Lo

doi:10.1039/c5ob00643k

Author(s)

D. Xu
L. Guo
Y. Chen
M. S. Wong

Detail(s)

Original language	English
Pages (from-to)	7307-7312
Journal / Publication	Organic and Biomolecular Chemistry
Volume	13
Issue number	26
Publication status	Published - 14 Jul 2015

Link(s)

DOI	DOI https://doi.org/10.1039/c5ob00643k Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-84934916661&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(07cfc553-aed0-4ff0-a9f6-343af7019403).html

Abstract

We report the first example of a novel two-photon active, biocompatible, and macrophage cell-membrane permeable carbazole-based cyanine fluorophore for the detection of three biologically important ROS, namely, ^•OH, O₂^- and OCl^- in solution. This versatile probe shows cellular protection not only in stimulated macrophages from phorbol-12-myristate-13-acetate-induced morphological changes but also lipopolysaccharide-induced cytotoxicity by quenching with the O₂^- and OCl^- production, respectively. Such protection could be visualized by a distinct change in the fluorescence intensity of the probe.

Citation Format(s)

[ RIS ] [ BibTeX ]

Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection. / Chan, M. S.; Xu, D.; Guo, L. et al.
In: Organic and Biomolecular Chemistry, Vol. 13, No. 26, 14.07.2015, p. 7307-7312.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Chan, MS, Xu, D, Guo, L, Tam, DY , Liu, LS, Chen, Y, Wong, MS & Lo, PK 2015, 'Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection', Organic and Biomolecular Chemistry, vol. 13, no. 26, pp. 7307-7312. https://doi.org/10.1039/c5ob00643k

Chan, M. S., Xu, D., Guo, L., Tam, D. Y., Liu, L. S., Chen, Y., Wong, M. S., & Lo, P. K. (2015). Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection. Organic and Biomolecular Chemistry, 13(26), 7307-7312. https://doi.org/10.1039/c5ob00643k

Chan MS, Xu D, Guo L, Tam DY , Liu LS, Chen Y et al. Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection. Organic and Biomolecular Chemistry. 2015 Jul 14;13(26):7307-7312. doi: 10.1039/c5ob00643k

Chan, M. S. ; Xu, D. ; Guo, L. et al. / Cyanine fluorophores for cellular protection against ROS in stimulated macrophages and two-photon ROS detection. In: Organic and Biomolecular Chemistry. 2015 ; Vol. 13, No. 26. pp. 7307-7312.