Cultivation of sulfide-driven partial denitrification granules for efficient nitrite generation from nitrate-sulfide-laden wastewater

Sulfide partial denitrification (SPD) is an alternative pathway for nitrite production accompanied with elemental sulfur (S⁰) production for nitrate removal from wastewater with anammox. In this study, the SPD granular sludge was cultivated for the first time in an upflow anaerobic sludge bed (UASB) reactor to reach the efficacy of maximum nitrate-to-nitrite transformation ratio of 92% and an in-situ maximum NO₃⁻-N reduction rate of 2.46 kg-N/m³-d, both much higher than literature results. Mature granules had an average particle size of 2.52 mm and hold smooth surface with excess rod bacteria. The elements Ca and S, and proteins in extracellular polymeric substances contributed to granule structure's stability. Enriched Thiobacillus genus was proposed to accumulate nitrite at moderate HRT (2–6 h). The immobilized functional strains assist efficient partial nitrification reactions to be realized with formed S⁰ as byproduct.