Cryomicroneedles for transdermal cell delivery

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

2 Scopus Citations
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Author(s)

  • Hao Chang
  • Sharon W. T. Chew
  • Mengjia Zheng
  • Daniel Chin Shiuan Lio
  • Christian Wiraja
  • Yu Mei
  • Xiaoyu Ning
  • Mingyue Cui
  • Aung Than
  • Kanyi Pu
  • Peng Chen
  • Haiyan Liu

Related Research Unit(s)

Detail(s)

Original languageEnglish
Journal / PublicationNature Biomedical Engineering
Online published3 May 2021
Publication statusOnline published - 3 May 2021

Abstract

Cell therapies for the treatment of skin disorders could benefit from simple, safe and efficient technology for the transdermal delivery of therapeutic cells. Conventional cell delivery by hypodermic-needle injection is associated with poor patient compliance, requires trained personnel, generates waste and has non-negligible risks of injury and infection. Here, we report the design and proof-of-concept application of cryogenic microneedle patches for the transdermal delivery of living cells. The microneedles are fabricated by stepwise cryogenic micromoulding of cryogenic medium with pre-suspended cells, and can be easily inserted into porcine skin and dissolve after deployment of the cells. In mice, cells delivered by the cryomicroneedles retained their viability and proliferative capability. In mice with subcutaneous melanoma tumours, the delivery of ovalbumin-pulsed dendritic cells via the cryomicroneedles elicited higher antigen-specific immune responses and led to slower tumour growth than intravenous and subcutaneous injections of the cells. Biocompatible cryomicroneedles may facilitate minimally invasive cell delivery for a range of cell therapies.

Citation Format(s)

Cryomicroneedles for transdermal cell delivery. / Chang, Hao; Chew, Sharon W. T.; Zheng, Mengjia; Lio, Daniel Chin Shiuan; Wiraja, Christian; Mei, Yu; Ning, Xiaoyu; Cui, Mingyue; Than, Aung; Shi, Peng; Wang, Dongan; Pu, Kanyi; Chen, Peng; Liu, Haiyan; Xu, Chenjie.

In: Nature Biomedical Engineering, 03.05.2021.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review