Critical Factors Facilitating Candidatus Nitrotoga to Be Prevalent Nitrite-Oxidizing Bacteria in Activated Sludge

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Min Zheng
  • Siqi Li
  • Gaofeng Ni
  • Jun Xia
  • Shihu Hu
  • Yanchen Liu
  • Xia Huang

Detail(s)

Original languageEnglish
Pages (from-to)15414-15423
Journal / PublicationEnvironmental Science and Technology
Volume54
Issue number23
Online published12 Nov 2020
Publication statusPublished - 1 Dec 2020
Externally publishedYes

Abstract

Nitrite oxidation is the primary pathway that generates nitrate in engineered systems. However, little is known about the role of a novel nitrite-oxidizing bacteria (NOB) genus Candidatus Nitrotoga in activated sludge systems. To elucidate key factors that impact NOB community composition, laboratory-scale sequencing batch reactors (SBRs) were designed and operated under the same conditions as real wastewater treatment plants to achieve considerable nitrogen removal and similar community; then, different conditions including temperature (T), dissolved oxygen (DO), free nitrous acid (FNA), and free ammonia (FA) were applied. The 16S rRNA gene-based PCR and sequence analysis illustrated that Ca. Nitrotoga were abundant even at ambient temperature, thus further challenging the previous conception of them being solely cold-adapted. Ca. Nitrotoga are less competitive than Nitrospira during oxygen deficiency, indicating its lower affinity to dissolved oxygen. Ca. Nitrotoga are the dominant nitrite oxidizers under regular exposure to FNA and FA due to their relatively higher resistance than other NOB toward these two effective biocides. Therefore, this study demonstrates that Ca. Nitrotoga can play an important role in biological nitrogen removal and also highlights the need for multiple strategies for NOB suppression for the next-generation, shortcut nitrogen removal. © American Chemical Society.

Citation Format(s)

Critical Factors Facilitating Candidatus Nitrotoga to Be Prevalent Nitrite-Oxidizing Bacteria in Activated Sludge. / Zheng, Min; Li, Siqi; Ni, Gaofeng et al.
In: Environmental Science and Technology, Vol. 54, No. 23, 01.12.2020, p. 15414-15423.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review