CRISPR/Cas9 screening using unique molecular identifiers

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

37 Scopus Citations
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Author(s)

  • Bernhard Schmierer
  • Sandeep K Botla
  • Mikko Turunen
  • Teemu Kivioja
  • Jussi Taipale

Detail(s)

Original languageEnglish
Article number945
Journal / PublicationMolecular Systems Biology
Volume13
Issue number10
Publication statusPublished - 1 Oct 2017
Externally publishedYes

Link(s)

Abstract

Loss-of-function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing and lineage dropout screening. RSLs greatly improve the reproducibility of results by increasing both the precision and the accuracy of screens. They reduce the number of cells needed to reach a set statistical power, or allow a more robust screen using the same number of cells.

Research Area(s)

  • CRISPR/Cas, genetic screening, massively parallel lineage tracing, unique molecular identifiers

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

CRISPR/Cas9 screening using unique molecular identifiers. / Schmierer, Bernhard; Botla, Sandeep K; Zhang, Jilin et al.
In: Molecular Systems Biology, Vol. 13, No. 10, 945, 01.10.2017.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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