CRISPR/Cas9 screening using unique molecular identifiers

Bernhard Schmierer; Sandeep K Botla; Jilin Zhang; Mikko Turunen; Teemu Kivioja; Jussi Taipale

doi:10.15252/msb.20177834

CRISPR/Cas9 screening using unique molecular identifiers

Bernhard Schmierer, Sandeep K Botla, Jilin Zhang, Mikko Turunen, Teemu Kivioja, Jussi Taipale^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

44 Citations (Scopus)

29 Downloads (CityUHK Scholars)

Abstract

Loss-of-function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing and lineage dropout screening. RSLs greatly improve the reproducibility of results by increasing both the precision and the accuracy of screens. They reduce the number of cells needed to reach a set statistical power, or allow a more robust screen using the same number of cells.

Original language	English
Article number	945
Journal	Molecular Systems Biology
Volume	13
Issue number	10
DOIs	https://doi.org/10.15252/msb.20177834
Publication status	Published - 1 Oct 2017
Externally published	Yes

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Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

CRISPR/Cas
genetic screening
massively parallel lineage tracing
unique molecular identifiers

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This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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10.15252/msb.20177834Licence: CC BY

96412771Final Published version, 1.02 MBLicence: CC BY

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AU - Zhang, Jilin

AU - Turunen, Mikko

AU - Kivioja, Teemu

AU - Taipale, Jussi

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CRISPR/Cas9 screening using unique molecular identifiers

Abstract

Bibliographical note

Research Keywords

Publisher's Copyright Statement

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