Covalently Linked Deoxyribonucleic Acid with Multi-walled Carbon Nanotubes: Synthesis and Characterization

Weiwei Chen, Changqing YI, Tzang Chi-Hung, Shuit-Tong Lee, Mengsu Yang

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 12 - Chapter in an edited book (Author)peer-review

Abstract

In this chapter, a multi-step protocol for covalently linking functionalized multi-walled carbon nanotubes (MWCNT) to deoxyribonucleic acid (DNA) oligonucleotides is provided. X-ray photoelectron spectroscopy (XPS) is used to characterize the initially formed amine-terminated MWCNTs, to which DNA is covalently anchored. Atomic force microscopy (AFM) investigation of the DNA-MWCNT conjugates reveals that the chemical functionalization occurs at both the ends and sidewalls of the nanotubes. The described methodology represents an important step toward the realization of DNA-guided self-assembly for carbon nanotubes.
Original languageEnglish
Title of host publicationCarbon Nanotubes
Subtitle of host publicationMethods and Protocols
EditorsKannan Balasubramanian, Marko Burghard
PublisherHumana Press
Pages19-26
ISBN (Electronic)978-1-60761-579-8
ISBN (Print)978-1-60761-577-4, 978-1-4939-5746-0
DOIs
Publication statusPublished - 2010

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume625
ISSN (Print)1064-3745

Research Keywords

  • Carbon nanotubes
  • Covalent conjugation
  • DNA
  • ATR-FTIR
  • XPS
  • AFM

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