Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

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Author(s)

  • D. R. Glenn
  • H. Zhang
  • N. Kasthuri
  • R. Schalek
  • A. S. Trifonov
  • H. Park
  • J. W. Lichtman
  • R. L. Walsworth

Detail(s)

Original languageEnglish
Article number865
Journal / PublicationScientific Reports
Volume2
Publication statusPublished - 2012
Externally publishedYes

Abstract

Correlative light and electron microscopy promises to combine molecular specificity with nanoscale imaging resolution. However, there are substantial technical challenges including reliable co-registration of optical and electron images, and rapid optical signal degradation under electron beam irradiation. Here, we introduce a new approach to solve these problems: imaging of stable optical cathodoluminescence emitted in a scanning electron microscope by nanoparticles with controllable surface chemistry. We demonstrate well-correlated cathodoluminescence and secondary electron images using three species of semiconductor nanoparticles that contain defects providing stable, spectrally-distinguishable cathodoluminescence. We also demonstrate reliable surface functionalization of the particles. The results pave the way for the use of such nanoparticles for targeted labeling of surfaces to provide nanoscale mapping of molecular composition, indicated by cathodoluminescence colour, simultaneously acquired with structural electron images in a single instrument. © 2012 Macmillan Publishers Limited. All rights reserved.

Citation Format(s)

Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours. / Glenn, D. R.; Zhang, H.; Kasthuri, N.; Schalek, R.; Lo, P. K.; Trifonov, A. S.; Park, H.; Lichtman, J. W.; Walsworth, R. L.

In: Scientific Reports, Vol. 2, 865, 2012.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review