Correlation of F4-neuroprostanes levels in cerebrospinal fluid with outcome of aneurysmal subarachnoid hemorrhage in humans

Yu-Ping Hsieh; Chih-Lung Lin; An-Li Shiue; Huiyong Yin; Jason D. Morrow; Jee-Ching Hsu; Tsung-Che Hsieh; Hsing-Ju Wei; Hsiu-Chuan Yen

doi:10.1016/j.freeradbiomed.2009.06.026

Correlation of F₄-neuroprostanes levels in cerebrospinal fluid with outcome of aneurysmal subarachnoid hemorrhage in humans

Yu-Ping Hsieh
, Chih-Lung Lin
, An-Li Shiue
, Huiyong Yin
, Jason D. Morrow
, Jee-Ching Hsu
, Tsung-Che Hsieh
, Hsing-Ju Wei
, Hsiu-Chuan Yen

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

50 Citations (Scopus)

Abstract

Aneurysmal subarachnoid hemorrhage (aSAH) is one type of hemorrhagic stroke in humans. F₂-isoprostanes (F₂-IsoPs) and F₄-neuroprostanes (F₄-NPs), derived from arachidonic acid and docosahexaenoic acid (DHA), respectively, are specific markers of lipid peroxidation. We previously demonstrated that F₂-IsoPs levels in cerebrospinal fluid (CSF) of aSAH patients positively correlated with poor clinical conditions. In this work, we refined F₄-NPs analysis and investigated the role of potential oxidative damage to neurons in aSAH patients by detecting F₄-NPs in CSF. [²H₄]-15-F_2t-IsoP, rather than [¹⁸O₂]-17-F_4c-NP or [²H₄]-PGF_2α, was used as the internal standard for F₄-NPs analysis. One problem of the use of [¹⁸O₂]-17-F_4c-NP was the potential interference resulting from F₂-dihomo-IsoPs in CSF. CSF specimens of 15 aSAH patients for up to 10 days and those of 12 non-aSAH controls were analyzed. First day, mean, and peak levels of F₄-NPs were all significantly higher in aSAH patients than in controls and correlated with the Fisher Scale and 3-month Glasgow Outcome Scale, but only mean levels of F₄-NPs correlated with Hunt and Hess Grade. The results first demonstrate oxidative damage to DHA in brain tissue following aSAH and suggest that F₄-NPs in CSF could be a better predictor for outcome of aSAH than F₂-IsoPs at early time points. © 2009 Elsevier Inc.

Original language	English
Pages (from-to)	814-824
Journal	Free Radical Biology and Medicine
Volume	47
Issue number	6
DOIs	https://doi.org/10.1016/j.freeradbiomed.2009.06.026
Publication status	Published - 15 Sept 2009
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

The corresponding author express the deepest appreciation to Dr. Jason D. Morrow for his great help on establishing GC/NICI-MS analysis for F2-IsoPs and F4-NPs and his inspiration since 2002. His sudden passing in 2008 grieved us all. This study was supported by grants NSC96-2320-B-182-018 and NSC97-2320-B-182-012-MY3 to H.-C. Yen from the National Science Council, Taiwan; and grants DK 48831, GM-15431, ES-13125 to H. Yin from the National Institutes of Health, USA. This study was also partially supported by Chang Gung Molecular Medicine Research Center at Chang Gung University with grants EMRPD160241 and EMRPD170581 from the Ministry of Education, Taiwan.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Aneurysmal subarachnoid hemorrhage
Cerebrospinal fluid
Docosahexaenoic acid
F4-neuroprostanes
Lipid peroxidation
Outcome

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title = "Correlation of F4-neuroprostanes levels in cerebrospinal fluid with outcome of aneurysmal subarachnoid hemorrhage in humans",

abstract = "Aneurysmal subarachnoid hemorrhage (aSAH) is one type of hemorrhagic stroke in humans. F2-isoprostanes (F2-IsoPs) and F4-neuroprostanes (F4-NPs), derived from arachidonic acid and docosahexaenoic acid (DHA), respectively, are specific markers of lipid peroxidation. We previously demonstrated that F2-IsoPs levels in cerebrospinal fluid (CSF) of aSAH patients positively correlated with poor clinical conditions. In this work, we refined F4-NPs analysis and investigated the role of potential oxidative damage to neurons in aSAH patients by detecting F4-NPs in CSF. [2H4]-15-F2t-IsoP, rather than [18O2]-17-F4c-NP or [2H4]-PGF2α, was used as the internal standard for F4-NPs analysis. One problem of the use of [18O2]-17-F4c-NP was the potential interference resulting from F2-dihomo-IsoPs in CSF. CSF specimens of 15 aSAH patients for up to 10 days and those of 12 non-aSAH controls were analyzed. First day, mean, and peak levels of F4-NPs were all significantly higher in aSAH patients than in controls and correlated with the Fisher Scale and 3-month Glasgow Outcome Scale, but only mean levels of F4-NPs correlated with Hunt and Hess Grade. The results first demonstrate oxidative damage to DHA in brain tissue following aSAH and suggest that F4-NPs in CSF could be a better predictor for outcome of aSAH than F2-IsoPs at early time points. {\textcopyright} 2009 Elsevier Inc.",

keywords = "Aneurysmal subarachnoid hemorrhage, Cerebrospinal fluid, Docosahexaenoic acid, F4-neuroprostanes, Lipid peroxidation, Outcome",

author = "Yu-Ping Hsieh and Chih-Lung Lin and An-Li Shiue and Huiyong Yin and Morrow, \{Jason D.\} and Jee-Ching Hsu and Tsung-Che Hsieh and Hsing-Ju Wei and Hsiu-Chuan Yen",

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T1 - Correlation of F4-neuroprostanes levels in cerebrospinal fluid with outcome of aneurysmal subarachnoid hemorrhage in humans

AU - Hsieh, Yu-Ping

AU - Lin, Chih-Lung

AU - Shiue, An-Li

AU - Yin, Huiyong

AU - Morrow, Jason D.

AU - Hsu, Jee-Ching

AU - Hsieh, Tsung-Che

AU - Wei, Hsing-Ju

AU - Yen, Hsiu-Chuan

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2009/9/15

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N2 - Aneurysmal subarachnoid hemorrhage (aSAH) is one type of hemorrhagic stroke in humans. F2-isoprostanes (F2-IsoPs) and F4-neuroprostanes (F4-NPs), derived from arachidonic acid and docosahexaenoic acid (DHA), respectively, are specific markers of lipid peroxidation. We previously demonstrated that F2-IsoPs levels in cerebrospinal fluid (CSF) of aSAH patients positively correlated with poor clinical conditions. In this work, we refined F4-NPs analysis and investigated the role of potential oxidative damage to neurons in aSAH patients by detecting F4-NPs in CSF. [2H4]-15-F2t-IsoP, rather than [18O2]-17-F4c-NP or [2H4]-PGF2α, was used as the internal standard for F4-NPs analysis. One problem of the use of [18O2]-17-F4c-NP was the potential interference resulting from F2-dihomo-IsoPs in CSF. CSF specimens of 15 aSAH patients for up to 10 days and those of 12 non-aSAH controls were analyzed. First day, mean, and peak levels of F4-NPs were all significantly higher in aSAH patients than in controls and correlated with the Fisher Scale and 3-month Glasgow Outcome Scale, but only mean levels of F4-NPs correlated with Hunt and Hess Grade. The results first demonstrate oxidative damage to DHA in brain tissue following aSAH and suggest that F4-NPs in CSF could be a better predictor for outcome of aSAH than F2-IsoPs at early time points. © 2009 Elsevier Inc.

AB - Aneurysmal subarachnoid hemorrhage (aSAH) is one type of hemorrhagic stroke in humans. F2-isoprostanes (F2-IsoPs) and F4-neuroprostanes (F4-NPs), derived from arachidonic acid and docosahexaenoic acid (DHA), respectively, are specific markers of lipid peroxidation. We previously demonstrated that F2-IsoPs levels in cerebrospinal fluid (CSF) of aSAH patients positively correlated with poor clinical conditions. In this work, we refined F4-NPs analysis and investigated the role of potential oxidative damage to neurons in aSAH patients by detecting F4-NPs in CSF. [2H4]-15-F2t-IsoP, rather than [18O2]-17-F4c-NP or [2H4]-PGF2α, was used as the internal standard for F4-NPs analysis. One problem of the use of [18O2]-17-F4c-NP was the potential interference resulting from F2-dihomo-IsoPs in CSF. CSF specimens of 15 aSAH patients for up to 10 days and those of 12 non-aSAH controls were analyzed. First day, mean, and peak levels of F4-NPs were all significantly higher in aSAH patients than in controls and correlated with the Fisher Scale and 3-month Glasgow Outcome Scale, but only mean levels of F4-NPs correlated with Hunt and Hess Grade. The results first demonstrate oxidative damage to DHA in brain tissue following aSAH and suggest that F4-NPs in CSF could be a better predictor for outcome of aSAH than F2-IsoPs at early time points. © 2009 Elsevier Inc.

KW - Aneurysmal subarachnoid hemorrhage

KW - Cerebrospinal fluid

KW - Docosahexaenoic acid

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KW - Lipid peroxidation

KW - Outcome

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