Core-Shell Droplet-Based Angiogenic Patches for the Treatment of Ischemic Diseases : Ultrafast Processability, Physical Tunability, and Controlled Delivery of an Angiogenic Cocktail

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

1 Scopus Citations
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Author(s)

  • Jin-Ju Kim
  • Xin Li
  • Seok Beom Hong
  • Yeon-Jik Choi
  • Hae-Won Kim
  • Hun-Jun Park

Detail(s)

Original languageEnglish
Pages (from-to)50693–50707
Number of pages15
Journal / PublicationACS Applied Materials & Interfaces
Volume15
Issue number44
Online published9 Oct 2023
Publication statusPublished - 8 Nov 2023

Abstract

The patch-based delivery system has been a promising therapeutic approach for treating various vascular diseases. However, conventional methods face several challenges, including labor-intensive and time-consuming processes associated with patch fabrication or factor incorporation, inadequate physical properties, and uncontrolled release of factors. These limitations restrict the potential applications in clinical settings. To overcome these issues, we propose a novel core-shell-shaped droplet patch system called an angiogenic patch (AP). Our system offers several distinct advantages over conventional patches. It enables a rapid and straightforward fabrication process utilizing only two biodegradable ingredients [alginate and ε-poly(L-lysine)], ensuring minimal toxicity. Moreover, the AP exhibits excellent physical integrity to match and withstand physiological mechanics and allows for customizable patch dimensions tailored to individual patients' pathological conditions. Notably, the AP enables facile loading of angiogenic cytokines during patch fabrication, allowing sustained release at a controlled rate through tunable network cross-linking. Subsequently, the AP, delivering a precisely formulated cocktail of angiogenic cytokines (VEGF, bFGF, EGF, and IGF), demonstrated significant effects on endothelial cell functions (migration and tubule formation) and survival under pathological conditions simulating ischemic injury. Likewise, in in vivo experiments using a mouse model of hindlimb ischemia, the AP encapsulating the angiogenic cocktail effectively restored blood flow following an ischemic insult, promoting muscle regeneration and preventing limb loss. With its simplicity and rapid processability, user-friendly applicability, physical tunability, and the ability to efficiently load and control the delivery of angiogenic factors, the AP holds great promise as a therapeutic means for treating patients with ischemic diseases. © 2023 American Chemical Society.


Research Area(s)

  • patch-based delivery system, core−shell droplet, ultrafast processing, angiogenic cocktail, ischemic disease, vascular regeneration

Citation Format(s)

Core-Shell Droplet-Based Angiogenic Patches for the Treatment of Ischemic Diseases: Ultrafast Processability, Physical Tunability, and Controlled Delivery of an Angiogenic Cocktail. / Bui, Thi Van Anh; Kim, Jin-Ju; Huang, Xin et al.
In: ACS Applied Materials & Interfaces, Vol. 15, No. 44, 08.11.2023, p. 50693–50707.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review