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Coordinate-based neural representations for computational adaptive optics in two-photon microscopy

  • Iksung Kang
  • , Qinrong Zhang
  • , Courtney Yaeger
  • , Trang Pham
  • , Stella X. Yu
  • , Mark Harnett
  • , Na Ji

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

Abstract

By measuring and correcting sample-induced aberrations, adaptive optics (AO) enables noninvasive imaging of subcellular structures in living organisms with two-photon (2P) fluorescence microscopy. We will introduce CoCoA-2P, a self-supervised machine-learning algorithm capable of simultaneously estimating aberrations and recovering 3D structural information from a single 2P image stack without requiring external training datasets. We will showcase the applications of CoCoA-2P for high-resolution in vivo structural imaging of the mouse brain and eye lenses. © 2024 SPIE.
Original languageEnglish
Title of host publicationAdaptive Optics and Wavefront Control for Biological Systems X
EditorsThomas G. Bifano, Na Ji, Lei Tian
PublisherSPIE - International Society for Optical Engineering
ISBN (Electronic)9781510669628
ISBN (Print)9781510669611
DOIs
Publication statusPublished - 2024
Externally publishedYes
EventSPIE BIOS 2024 - San Francisco, United States
Duration: 28 Jan 202429 Jan 2024

Publication series

NameProceedings of SPIE
Volume12851
ISSN (Print)1605-7422
ISSN (Electronic)2410-9045

Conference

ConferenceSPIE BIOS 2024
PlaceUnited States
CitySan Francisco
Period28/01/2429/01/24

Research Keywords

  • Adaptive optics
  • Machine learning
  • Two-photon microscopy

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