Controlled chondrogenesis from adipose-derived stem cells by recombinant transforming growth factor-β3 fusion protein in peptide scaffolds

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Dong Zheng
  • Yang Dan
  • Shu-hua Yang
  • Guo-hui Liu
  • Zeng-wu Shao
  • Cao Yang
  • Bao-jun Xiao
  • Xiangmei Liu
  • Shuilin Wu
  • Tainjin Zhang

Detail(s)

Original languageEnglish
Pages (from-to)191-203
Journal / PublicationActa Biomaterialia
Volume11
Online published23 Sept 2014
Publication statusPublished - 1 Jan 2015

Abstract

Adipose-derived stem cells (ADSCs) are promising for cartilage repair due to their easy accessibility and chondrogenic potential. Although chondrogenesis of transforming growth factor-β (TGF-β) mediated mesenchymal stem cells (MSCs) is well established in vitro, clinical tissue engineering requires effective and controlled delivery of TGF-β in vivo. In this work, a self-assembled peptide scaffold was employed to construct cartilages in vivo through the chondrogenesis from ADSCs controlled by recombinant fusion protein LAP-MMP-mTGF-β3 that was transfected by lentiviral vectors. During this course, the addition of matrix metalloproteinases (MMPs) can trigger the release of mTGF-β3 from the recombinant fusion protein of LAP-MMP-mTGF-β3 in the combined scaffolds, thus stimulating the differentiation of ADSCs into chondrogenesis. The specific expression of cartilage genes was analyzed by real-time polymerase chain reaction and Western blot. The expression of chondrocytic markers was obviously upregulated to a higher level compared to the one by commonly used TGF-β3 alone. After 3 weeks of in vitro culturing, the hybrids with differentiated chondrogenesis were then injected subcutaneously into nude mice and retrieved after 4 weeks of culturing in vivo. Histological analysis also confirmed that the recombinant fusion protein was more effective for the formation of cartilage matrix than the cases either with TGF-β3 alone or without LAP-MMP-mTGF-β3 (P <0.05). This study demonstrates that controlled local delivery of the LAP-MMP-mTGF-β3 constructs can accelerate differentiation of ADSCs into the cartilage in vivo, which indicates the great potential of this hybrid in rapid therapy of osteoarthritis. © 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Research Area(s)

  • Adipose-derived stem cells, Cartilage, Hydrogel, Osteoarthritis, Scaffolds

Citation Format(s)

Controlled chondrogenesis from adipose-derived stem cells by recombinant transforming growth factor-β3 fusion protein in peptide scaffolds. / Zheng, Dong; Dan, Yang; Yang, Shu-hua et al.
In: Acta Biomaterialia, Vol. 11, 01.01.2015, p. 191-203.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review