CONTINUOUS FLOW MICROFLUIDIC SYSTEM FOR CUSTOMIZABLE BACTERIAL CELLULOSE PRODUCTION THROUGH SYNTHETIC BIOLOGY

Jiaqing Yu; Guoyun Sun; Myat Noe Hsu; Vishnu Vadanan Sundaravadanam; Sierin Lim; Chia-Hung Chen

CONTINUOUS FLOW MICROFLUIDIC SYSTEM FOR CUSTOMIZABLE BACTERIAL CELLULOSE PRODUCTION THROUGH SYNTHETIC BIOLOGY

Jiaqing Yu
, Guoyun Sun
, Myat Noe Hsu
, Vishnu Vadanan Sundaravadanam
, Sierin Lim
, Chia-Hung Chen^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

This paper presents a high-throughput continuous-flow microfluidic sorting system for customizable bacterial cellulose (BC) production. Gluconacetobacter xylinus was encapsulated in agarose hydrogel particle using droplet based microfluidics for a long-time static culture to generate BC. A real time image analysis system was then applied to distinguish the hydrogel particles according to their BC content by the light scattering pattern. This sorting device could select the target hydrogel particles containing bacteria capable of producing high BC volume for downstream genome analysis.

Original language	English
Title of host publication	μTAS 2017
Subtitle of host publication	The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	961-962
ISBN (Electronic)	9780692941836
Publication status	Published - Oct 2017
Externally published	Yes
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017) - Savannah, United States Duration: 22 Oct 2017 → 26 Oct 2017 https://archive.cbmsociety.org/utas/uTAS2017.zip

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
Publisher	Chemical and Biological Microsystems Society
ISSN (Electronic)	1556-5904

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
Place	United States
City	Savannah
Period	22/10/17 → 26/10/17
Internet address	https://archive.cbmsociety.org/utas/uTAS2017.zip

Research Keywords

Bacterial cellulose
Hydrogel
Image sorting
Microfluidic
Synthetic biology

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Yu, J., Sun, G., Hsu, M. N., Sundaravadanam, V. V., Lim, S., & Chen, C.-H. (2017). CONTINUOUS FLOW MICROFLUIDIC SYSTEM FOR CUSTOMIZABLE BACTERIAL CELLULOSE PRODUCTION THROUGH SYNTHETIC BIOLOGY. In μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 961-962). (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS). Chemical and Biological Microsystems Society.

Yu, Jiaqing ; Sun, Guoyun ; Hsu, Myat Noe et al. / CONTINUOUS FLOW MICROFLUIDIC SYSTEM FOR CUSTOMIZABLE BACTERIAL CELLULOSE PRODUCTION THROUGH SYNTHETIC BIOLOGY. μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2017. pp. 961-962 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

@inproceedings{026f4e32195d4c21836baa8843d2db6f,

title = "CONTINUOUS FLOW MICROFLUIDIC SYSTEM FOR CUSTOMIZABLE BACTERIAL CELLULOSE PRODUCTION THROUGH SYNTHETIC BIOLOGY",

abstract = "This paper presents a high-throughput continuous-flow microfluidic sorting system for customizable bacterial cellulose (BC) production. Gluconacetobacter xylinus was encapsulated in agarose hydrogel particle using droplet based microfluidics for a long-time static culture to generate BC. A real time image analysis system was then applied to distinguish the hydrogel particles according to their BC content by the light scattering pattern. This sorting device could select the target hydrogel particles containing bacteria capable of producing high BC volume for downstream genome analysis.",

keywords = "Bacterial cellulose, Hydrogel, Image sorting, Microfluidic, Synthetic biology",

author = "Jiaqing Yu and Guoyun Sun and Hsu, \{Myat Noe\} and Sundaravadanam, \{Vishnu Vadanan\} and Sierin Lim and Chia-Hung Chen",

year = "2017",

month = oct,

language = "English",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS",

publisher = "Chemical and Biological Microsystems Society",

pages = "961--962",

booktitle = "μTAS 2017",

note = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017) ; Conference date: 22-10-2017 Through 26-10-2017",

url = "https://archive.cbmsociety.org/utas/uTAS2017.zip",

}

Yu, J, Sun, G, Hsu, MN, Sundaravadanam, VV, Lim, S & Chen, C-H 2017, CONTINUOUS FLOW MICROFLUIDIC SYSTEM FOR CUSTOMIZABLE BACTERIAL CELLULOSE PRODUCTION THROUGH SYNTHETIC BIOLOGY. in μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS, Chemical and Biological Microsystems Society, pp. 961-962, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017), Savannah, Georgia, United States, 22/10/17.

CONTINUOUS FLOW MICROFLUIDIC SYSTEM FOR CUSTOMIZABLE BACTERIAL CELLULOSE PRODUCTION THROUGH SYNTHETIC BIOLOGY. / Yu, Jiaqing; Sun, Guoyun; Hsu, Myat Noe et al.
μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2017. p. 961-962 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - CONTINUOUS FLOW MICROFLUIDIC SYSTEM FOR CUSTOMIZABLE BACTERIAL CELLULOSE PRODUCTION THROUGH SYNTHETIC BIOLOGY

AU - Yu, Jiaqing

AU - Sun, Guoyun

AU - Hsu, Myat Noe

AU - Sundaravadanam, Vishnu Vadanan

AU - Lim, Sierin

AU - Chen, Chia-Hung

PY - 2017/10

Y1 - 2017/10

N2 - This paper presents a high-throughput continuous-flow microfluidic sorting system for customizable bacterial cellulose (BC) production. Gluconacetobacter xylinus was encapsulated in agarose hydrogel particle using droplet based microfluidics for a long-time static culture to generate BC. A real time image analysis system was then applied to distinguish the hydrogel particles according to their BC content by the light scattering pattern. This sorting device could select the target hydrogel particles containing bacteria capable of producing high BC volume for downstream genome analysis.

AB - This paper presents a high-throughput continuous-flow microfluidic sorting system for customizable bacterial cellulose (BC) production. Gluconacetobacter xylinus was encapsulated in agarose hydrogel particle using droplet based microfluidics for a long-time static culture to generate BC. A real time image analysis system was then applied to distinguish the hydrogel particles according to their BC content by the light scattering pattern. This sorting device could select the target hydrogel particles containing bacteria capable of producing high BC volume for downstream genome analysis.

KW - Bacterial cellulose

KW - Hydrogel

KW - Image sorting

KW - Microfluidic

KW - Synthetic biology

UR - https://www.scopus.com/pages/publications/85079665225

UR - https://www.scopus.com/pages/publications/85079665225#tab=impact

M3 - RGC 32 - Refereed conference paper (with host publication)

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

SP - 961

EP - 962

BT - μTAS 2017

PB - Chemical and Biological Microsystems Society

T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)

Y2 - 22 October 2017 through 26 October 2017

ER -

Yu J, Sun G, Hsu MN, Sundaravadanam VV, Lim S, Chen CH. CONTINUOUS FLOW MICROFLUIDIC SYSTEM FOR CUSTOMIZABLE BACTERIAL CELLULOSE PRODUCTION THROUGH SYNTHETIC BIOLOGY. In μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2017. p. 961-962. (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

CONTINUOUS FLOW MICROFLUIDIC SYSTEM FOR CUSTOMIZABLE BACTERIAL CELLULOSE PRODUCTION THROUGH SYNTHETIC BIOLOGY

Abstract

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Research Keywords

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