Continuous 17α-ethinylestradiol exposure impairs the sperm quality of marine medaka (Oryzias melastigma)

Xian Qin; Keng Po Lai; Rudolf Shiu Sun Wu; Richard Yuen Chong Kong

doi:10.1016/j.marpolbul.2022.114093

Continuous 17α-ethinylestradiol exposure impairs the sperm quality of marine medaka (Oryzias melastigma)

Xian Qin, Keng Po Lai^*, Rudolf Shiu Sun Wu, Richard Yuen Chong Kong^*

^*Corresponding author for this work

Department of Chemistry

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

8 Citations (Scopus)

Abstract

17α-ethinylestradiol (EE2) is an anthropogenic estrogen that is widely used for hormone therapy and oral contraceptives. It was reported that EE2 exposure induced reproductive impairments through processes affecting reproduction behavior and inducing ovotestis. However, the effects of continuous EE2 exposure on the reproductive performance remain largely unknown. In this study, adult marine medaka fish (Oryzias melastigma) were exposed to EE2 (85 ng/L) for one (F₀) and two (F₁) generations. Our results indicate that continuous EE2 exposure reduced fecundity and sperm motility. The testicular transcriptome, followed by bioinformatic analysis revealed the dysregulation of pathways related to steroidogenesis, sperm motility, and reproductive system development. Collectively, our findings indicate that continuous EE2 exposure directly affected sperm quality via the alteration of steroidogenesis and dysregulation of reproductive system development. The identified key factors including DNM1, PINK1, PDE7B, and SLC12A7 can serve as biomarkers to assess EE2-reduced sperm motility.

Original language	English
Article number	114093
Journal	Marine Pollution Bulletin
Volume	183
Online published	7 Sept 2022
DOIs	https://doi.org/10.1016/j.marpolbul.2022.114093
Publication status	Published - Oct 2022

Funding

This work was financially supported by a General Research Fund (CityU 11104520) to RYCK and a CRF grant (SKLMP/CRF/0004) of the State Key Laboratory of Marine Pollution of the City University of Hong Kong to RSSW.

Research Keywords

Biomarkers
Ethinylestradiol
Fish
Multigeneration
Reproduction
Sperm quality

RGC Funding Information

RGC-funded

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.marpolbul.2022.114093

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@article{ee6a420199bf47d68c4ae95567c22b88,

title = "Continuous 17α-ethinylestradiol exposure impairs the sperm quality of marine medaka (Oryzias melastigma)",

abstract = "17α-ethinylestradiol (EE2) is an anthropogenic estrogen that is widely used for hormone therapy and oral contraceptives. It was reported that EE2 exposure induced reproductive impairments through processes affecting reproduction behavior and inducing ovotestis. However, the effects of continuous EE2 exposure on the reproductive performance remain largely unknown. In this study, adult marine medaka fish (Oryzias melastigma) were exposed to EE2 (85 ng/L) for one (F0) and two (F1) generations. Our results indicate that continuous EE2 exposure reduced fecundity and sperm motility. The testicular transcriptome, followed by bioinformatic analysis revealed the dysregulation of pathways related to steroidogenesis, sperm motility, and reproductive system development. Collectively, our findings indicate that continuous EE2 exposure directly affected sperm quality via the alteration of steroidogenesis and dysregulation of reproductive system development. The identified key factors including DNM1, PINK1, PDE7B, and SLC12A7 can serve as biomarkers to assess EE2-reduced sperm motility.",

keywords = "Biomarkers, Ethinylestradiol, Fish, Multigeneration, Reproduction, Sperm quality",

author = "Xian Qin and Lai, {Keng Po} and Wu, {Rudolf Shiu Sun} and Kong, {Richard Yuen Chong}",

year = "2022",

month = oct,

doi = "10.1016/j.marpolbul.2022.114093",

language = "English",

volume = "183",

journal = "Marine Pollution Bulletin",

issn = "0025-326X",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Continuous 17α-ethinylestradiol exposure impairs the sperm quality of marine medaka (Oryzias melastigma)

AU - Qin, Xian

AU - Lai, Keng Po

AU - Wu, Rudolf Shiu Sun

AU - Kong, Richard Yuen Chong

PY - 2022/10

Y1 - 2022/10

N2 - 17α-ethinylestradiol (EE2) is an anthropogenic estrogen that is widely used for hormone therapy and oral contraceptives. It was reported that EE2 exposure induced reproductive impairments through processes affecting reproduction behavior and inducing ovotestis. However, the effects of continuous EE2 exposure on the reproductive performance remain largely unknown. In this study, adult marine medaka fish (Oryzias melastigma) were exposed to EE2 (85 ng/L) for one (F0) and two (F1) generations. Our results indicate that continuous EE2 exposure reduced fecundity and sperm motility. The testicular transcriptome, followed by bioinformatic analysis revealed the dysregulation of pathways related to steroidogenesis, sperm motility, and reproductive system development. Collectively, our findings indicate that continuous EE2 exposure directly affected sperm quality via the alteration of steroidogenesis and dysregulation of reproductive system development. The identified key factors including DNM1, PINK1, PDE7B, and SLC12A7 can serve as biomarkers to assess EE2-reduced sperm motility.

AB - 17α-ethinylestradiol (EE2) is an anthropogenic estrogen that is widely used for hormone therapy and oral contraceptives. It was reported that EE2 exposure induced reproductive impairments through processes affecting reproduction behavior and inducing ovotestis. However, the effects of continuous EE2 exposure on the reproductive performance remain largely unknown. In this study, adult marine medaka fish (Oryzias melastigma) were exposed to EE2 (85 ng/L) for one (F0) and two (F1) generations. Our results indicate that continuous EE2 exposure reduced fecundity and sperm motility. The testicular transcriptome, followed by bioinformatic analysis revealed the dysregulation of pathways related to steroidogenesis, sperm motility, and reproductive system development. Collectively, our findings indicate that continuous EE2 exposure directly affected sperm quality via the alteration of steroidogenesis and dysregulation of reproductive system development. The identified key factors including DNM1, PINK1, PDE7B, and SLC12A7 can serve as biomarkers to assess EE2-reduced sperm motility.

KW - Biomarkers

KW - Ethinylestradiol

KW - Fish

KW - Multigeneration

KW - Reproduction

KW - Sperm quality

UR - http://www.scopus.com/inward/record.url?scp=85137295132&partnerID=8YFLogxK

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85137295132&origin=recordpage

U2 - 10.1016/j.marpolbul.2022.114093

DO - 10.1016/j.marpolbul.2022.114093

M3 - RGC 21 - Publication in refereed journal

C2 - 36084614

SN - 0025-326X

VL - 183

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

M1 - 114093

ER -

Continuous 17α-ethinylestradiol exposure impairs the sperm quality of marine medaka (Oryzias melastigma)

Abstract

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UN SDGs

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GRF: Interactive Effects of 17α-ethinylestradiol (EE2) and Hypoxia on Impairment of Testicular Functions in Male Marine Medaka Fish

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Continuous 17α-ethinylestradiol exposure impairs the sperm quality of marine medaka (Oryzias melastigma)

Abstract

Funding

Research Keywords

RGC Funding Information

UN SDGs

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GRF: Interactive Effects of 17α-ethinylestradiol (EE2) and Hypoxia on Impairment of Testicular Functions in Male Marine Medaka Fish

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