Abstract
In the past two to three decades, synthetic glycoconjugate vaccines have shown great potential in the prevention of severe infections and protection of high-risk populations. Conjugation of synthetic oligosaccharide haptens to carrier proteins is the key step for the vaccine preparation. In this review, the conjugation methods currently used in the synthesis of glycoconjugate vaccines from synthetic/homogeneous oligosaccharide haptens are summarized with the focus on the reaction conditions (pH and sugar/protein ratio) and performance. This information can help researchers choose the appropriate conjugation methods. Further research directions toward site-specific conjugations and fully homogeneous glycoconjugate vaccines are also discussed. © 2024 The Author(s). Chemistry - Methods published by Chemistry Europe and Wiley-VCH GmbH.
| Original language | English |
|---|---|
| Article number | e202300054 |
| Journal | Chemistry-Methods |
| Volume | 4 |
| Issue number | 7-8 |
| Online published | 26 Jul 2024 |
| DOIs | |
| Publication status | Published - Aug 2024 |
Funding
This work was supported by the Research Grant Council of Hong Kong (General Research Fund 17312822) and Health and Medical Research Fund (20190802, 22210712).
Research Keywords
- Conjugation
- Glycoconjugates
- Vaccine
- Oligosaccharides
- Carrier protein
Publisher's Copyright Statement
- This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/
RGC Funding Information
- RGC-funded