Conjugating Methotrexate to magnetite (Fe3O4) nanoparticles via trichloro-s-triazine

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)6400-6406
Journal / PublicationJournal of Materials Chemistry
Volume19
Issue number35
Online published1 Jul 2009
Publication statusPublished - 21 Sep 2009
Externally publishedYes

Abstract

Monodisperse Fe3O4 nanoparticles (NPs) originally synthesized with a hydrophobic oleylamine capping ligand were made water soluble and conjugated to the anticancer drug Methotrexate (MTX) using a new chemistry based on the readily available linker trichloro-s-triazine (TsT). This new linker is much more versatile than those that currently exist for attaching biomolecules to magnetic NPs. The MTX-conjugated NPs were found to be stable under physiological conditions for over 72 hours and MTX was shown to maintain its anticancer activity after conjugation to the NP surface. Through cell viability studies and intracellular uptake studies, MTX-conjugated NPs were shown to have targeting specificity for a tumor cell line (9L rat glioma) over a healthy cell line (Cultured Pulmonary Artery Endothelial). Additionally the MTX-conjugated NPs were visualized inside 9L cells using fluorescence microscopy to help elucidate their path within a cell after internalization. © 2009 The Royal Society of Chemistry.

Citation Format(s)

Conjugating Methotrexate to magnetite (Fe3O4) nanoparticles via trichloro-s-triazine. / Young, Kaylie L.; Xu, Chenjie; Xie, Jin; Sun, Shouheng.

In: Journal of Materials Chemistry, Vol. 19, No. 35, 21.09.2009, p. 6400-6406.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review