Concentration-dependent requirement for local protein synthesis in motor neuron subtype-specific response to axon guidance cues

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Stéphane Nédelec
  • Mirza Peljto
  • Mackenzie W. Amoroso
  • Lance C. Kam
  • Hynek Wichterle

Detail(s)

Original languageEnglish
Pages (from-to)1496-1506
Journal / PublicationJournal of Neuroscience
Volume32
Issue number4
Publication statusPublished - 25 Jan 2012

Abstract

Formation of functional motor circuits relies on the ability of distinct spinal motor neuron subtypes to project their axons with high precision to appropriate muscle targets. While guidance cues contributing to motor axon pathfinding have been identified, the intracellular pathways underlying subtype-specific responses to these cues remain poorly understood. In particular, it remains controversial whether responses to axon guidance cues depend on axonal protein synthesis. Using a growth cone collapse assay, we demonstrate that mouse embryonic stem cell-derived spinal motor neurons (ES-MNs) respond to ephrin-A5, Sema3f, and Sema3a in a concentrationdependent manner. At low doses, ES-MNs exhibit segmental or subtype-specific responses, while this selectivity is lost at higher concentrations. Response to high doses of semaphorins and to all doses of ephrin-A5 is protein synthesis independent. In contrast, using microfluidic devices and stripe assays, we show that growth cone collapse and guidance at low concentrations of semaphorins rely on local protein synthesis in the axonal compartment. Similar bimodal response to low and high concentrations of guidance cues is observed inhumanES-MNs, pointing to a general mechanism by which neurons increase their repertoire of responses to the limited set of guidance cues involved in neural circuit formation. © 2012 the authors.

Citation Format(s)

Concentration-dependent requirement for local protein synthesis in motor neuron subtype-specific response to axon guidance cues. / Nédelec, Stéphane; Peljto, Mirza; Shi, Peng; Amoroso, Mackenzie W.; Kam, Lance C.; Wichterle, Hynek.

In: Journal of Neuroscience, Vol. 32, No. 4, 25.01.2012, p. 1496-1506.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal