Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Qianli Ma
  • Wei Wang
  • Shenglin Mei
  • Kun Ji
  • Lei Jin
  • Yumei Zhang

Detail(s)

Original languageEnglish
Pages (from-to)1965-1976
Journal / PublicationInternational Journal of Nanomedicine
Volume7
Publication statusPublished - 2012

Link(s)

Abstract

Background: Titanium (Ti) implants are widely used clinically, but peri-implantitis remains one of the most common and serious complications. Healthy integration between gingival tissue and the implant surface is critical to long-term success in dental implant therapy. The objective of this study was to investigate how different concentrations of immobilized fibroblast growth factor 2 (FGF2) on the titania nanotubular surface influence the response of human gingival fibroblasts (HGFs). Methods: Pure Ti metal was anodized at 20 V to form a vertically organized titanium dioxide nanotube array on which three concentrations of FGF2 (250 ng/mL, 500 ng/mL, or 1000 ng/mL) were immobilized by repeated lyophilization. Surface topography was observed and FGF2 elution was detected using enzyme-linked immunosorbent assay. The bioactivity changes of dissolvable immobilized FGF2 were measured by methyl-thiazolyl-tetrazolium assay. Behavior of HGFs was evaluated using adhesion and methyl-thiazolyl-tetrazolium bromide assays. Results: The FGF2 remained for several days on the modified surface on which HGFs were cultured. Over 90% of the dissolvable immobilized FGF2 had been eluted by Day 9, whereas the FGF2 activity was found to diminish gradually from Day 1 to Day 9. The titania nanotubular surface with an optimal preparing concentration (500 ng/mL) of FGF2 immobilization exhibited improved HGF functions such as cellular attachment, proliferation, and extracellular matrix- related gene expression. Moreover, significant bidirectional as well as concentration- and time-dependent bioactivity was observed. Conclusion: Synergism of the FGF2-impregnated titanium dioxide nanotubular surface revealed good gingival-implant integration, indicating that these materials might have promising applications in dentistry and other biomedical devices. © 2012 Ma et al, publisher and licensee Dove Medical Press Ltd.

Research Area(s)

  • Dental implants, Extracellular matrix, Fibroblast growth factor 2, Real-time polymerase chain reaction, Titanium dioxide nanotube

Citation Format(s)

Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants. / Ma, Qianli; Wang, Wei; Chu, Paul K. et al.
In: International Journal of Nanomedicine, Vol. 7, 2012, p. 1965-1976.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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