Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 1965-1976 |
Journal / Publication | International Journal of Nanomedicine |
Volume | 7 |
Publication status | Published - 2012 |
Link(s)
DOI | DOI |
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Attachment(s) | Documents
Publisher's Copyright Statement
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-84866753873&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(d125270d-614c-4b5b-831e-d5073b8898dd).html |
Abstract
Background: Titanium (Ti) implants are widely used clinically, but peri-implantitis remains one of the most common and serious complications. Healthy integration between gingival tissue and the implant surface is critical to long-term success in dental implant therapy. The objective of this study was to investigate how different concentrations of immobilized fibroblast growth factor 2 (FGF2) on the titania nanotubular surface influence the response of human gingival fibroblasts (HGFs). Methods: Pure Ti metal was anodized at 20 V to form a vertically organized titanium dioxide nanotube array on which three concentrations of FGF2 (250 ng/mL, 500 ng/mL, or 1000 ng/mL) were immobilized by repeated lyophilization. Surface topography was observed and FGF2 elution was detected using enzyme-linked immunosorbent assay. The bioactivity changes of dissolvable immobilized FGF2 were measured by methyl-thiazolyl-tetrazolium assay. Behavior of HGFs was evaluated using adhesion and methyl-thiazolyl-tetrazolium bromide assays. Results: The FGF2 remained for several days on the modified surface on which HGFs were cultured. Over 90% of the dissolvable immobilized FGF2 had been eluted by Day 9, whereas the FGF2 activity was found to diminish gradually from Day 1 to Day 9. The titania nanotubular surface with an optimal preparing concentration (500 ng/mL) of FGF2 immobilization exhibited improved HGF functions such as cellular attachment, proliferation, and extracellular matrix- related gene expression. Moreover, significant bidirectional as well as concentration- and time-dependent bioactivity was observed. Conclusion: Synergism of the FGF2-impregnated titanium dioxide nanotubular surface revealed good gingival-implant integration, indicating that these materials might have promising applications in dentistry and other biomedical devices. © 2012 Ma et al, publisher and licensee Dove Medical Press Ltd.
Research Area(s)
- Dental implants, Extracellular matrix, Fibroblast growth factor 2, Real-time polymerase chain reaction, Titanium dioxide nanotube
Citation Format(s)
Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants. / Ma, Qianli; Wang, Wei; Chu, Paul K. et al.
In: International Journal of Nanomedicine, Vol. 7, 2012, p. 1965-1976.
In: International Journal of Nanomedicine, Vol. 7, 2012, p. 1965-1976.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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