Computational design of peptide-Au cluster probe for sensitive detection of αiIbβ3 integrin

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

10 Scopus Citations
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Author(s)

  • Lina Zhao
  • Xuejie Zhang
  • Xueyun Gao
  • Xiaohong Fang
  • Jingyuan Li

Detail(s)

Original languageEnglish
Pages (from-to)4203-4208
Journal / PublicationNanoscale
Volume8
Issue number7
Online published25 Jan 2016
Publication statusPublished - 21 Feb 2016
Externally publishedYes

Abstract

We have designed a novel peptide-Au cluster probe to specifically bind to αIIbβ3 integrin. As indicated by molecular dynamics (MD) simulations, the binding mode of the native ligand of αIIbβ3 integrin, γC peptide, can be realized by the designed probe. More importantly, the peptide-Au probe can provide multiple coating peptides to form additional salt bridges with protein, and the binding stability of the probe is comparable to the native ligand. The designed probe was then successfully synthesized. The specific binding in a cellular environment was validated by colocalization analysis of confocal microscopy. In addition, the binding affinity was confirmed by atomic force microscopy (AFM) based single molecule force spectroscopy. Our results suggest the combination of computational design and experimental verification can be a useful strategy for the development of nanoprobes.

Citation Format(s)

Computational design of peptide-Au cluster probe for sensitive detection of αiIbβ3 integrin. / Zhao, Lina; Zhai, Jiao; Zhang, Xuejie; Gao, Xueyun; Fang, Xiaohong; Li, Jingyuan.

In: Nanoscale, Vol. 8, No. 7, 21.02.2016, p. 4203-4208.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review