Comparison of three serological tests and an interferon-γ assay for the diagnosis of paratuberculosis in experimentally infected sheep

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • J. M. Gwozdz
  • K. G. Thompson
  • A. Murray
  • B. W. Manktelow
  • D. M. West

Detail(s)

Original languageEnglish
Pages (from-to)779-783
Journal / PublicationAustralian Veterinary Journal
Volume78
Issue number11
Publication statusPublished - Nov 2000
Externally publishedYes

Abstract

Objective To compare the diagnostic performance of a complement fixation test, an agar gel immunodiffusion test, an enzyme-linked immunosorbent assay, and a whole-blood interferon-γ assay for paratuberculosis in 14 sheep experimentally infected with Mycobacterium avium subsp paratuberculosis. Experimental design Longitudinal study. Results The IFN-γ assay detected more experimentally infected sheep, and earlier, than any of the serological tests. None of the antibody assays was able to detect all sheep with histologically confirmed paratuberculosis. Conclusions The superior performance of the IFN-γ assay in detecting infected sheep in this small experimental population warrants its further evaluation in a larger population of sheep naturally exposed to Ma paratuberculosis.

Research Area(s)

  • Interteron-γ, Paratuberculosis, Serology, Sheep

Citation Format(s)

Comparison of three serological tests and an interferon-γ assay for the diagnosis of paratuberculosis in experimentally infected sheep. / Gwozdz, J. M.; Thompson, K. G.; Murray, A.; Reichel, M. P.; Manktelow, B. W.; West, D. M.

In: Australian Veterinary Journal, Vol. 78, No. 11, 11.2000, p. 779-783.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal