Comparison of three serological tests and an interferon-γ assay for the diagnosis of paratuberculosis in experimentally infected sheep

J. M. Gwozdz; K. G. Thompson; A. Murray; M. P. Reichel; B. W. Manktelow; D. M. West

doi:10.1111/j.1751-0813.2000.tb10452.x

Author(s)

J. M. Gwozdz
K. G. Thompson
A. Murray
B. W. Manktelow
D. M. West

Detail(s)

Original language	English
Pages (from-to)	779-783
Journal / Publication	Australian Veterinary Journal
Volume	78
Issue number	11
Publication status	Published - Nov 2000
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1111/j.1751-0813.2000.tb10452.x Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-0034328516&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(0cd4e3da-13ba-4300-9496-79c7a7862ad6).html

Abstract

Objective To compare the diagnostic performance of a complement fixation test, an agar gel immunodiffusion test, an enzyme-linked immunosorbent assay, and a whole-blood interferon-γ assay for paratuberculosis in 14 sheep experimentally infected with Mycobacterium avium subsp paratuberculosis.

Experimental design Longitudinal study.

Results The IFN-γ assay detected more experimentally infected sheep, and earlier, than any of the serological tests. None of the antibody assays was able to detect all sheep with histologically confirmed paratuberculosis.

Conclusions The superior performance of the IFN-γ assay in detecting infected sheep in this small experimental population warrants its further evaluation in a larger population of sheep naturally exposed to Ma paratuberculosis.

Research Area(s)

Interteron-γ, Paratuberculosis, Serology, Sheep

Citation Format(s)

[ RIS ] [ BibTeX ]

Comparison of three serological tests and an interferon-γ assay for the diagnosis of paratuberculosis in experimentally infected sheep. / Gwozdz, J. M.; Thompson, K. G.; Murray, A. et al.
In: Australian Veterinary Journal, Vol. 78, No. 11, 11.2000, p. 779-783.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Gwozdz, JM, Thompson, KG, Murray, A, Reichel, MP, Manktelow, BW & West, DM 2000, 'Comparison of three serological tests and an interferon-γ assay for the diagnosis of paratuberculosis in experimentally infected sheep', Australian Veterinary Journal, vol. 78, no. 11, pp. 779-783. https://doi.org/10.1111/j.1751-0813.2000.tb10452.x

Gwozdz, J. M., Thompson, K. G., Murray, A., Reichel, M. P., Manktelow, B. W., & West, D. M. (2000). Comparison of three serological tests and an interferon-γ assay for the diagnosis of paratuberculosis in experimentally infected sheep. Australian Veterinary Journal, 78(11), 779-783. https://doi.org/10.1111/j.1751-0813.2000.tb10452.x

Gwozdz JM, Thompson KG, Murray A, Reichel MP, Manktelow BW, West DM. Comparison of three serological tests and an interferon-γ assay for the diagnosis of paratuberculosis in experimentally infected sheep. Australian Veterinary Journal. 2000 Nov;78(11):779-783. doi: 10.1111/j.1751-0813.2000.tb10452.x

Gwozdz, J. M. ; Thompson, K. G. ; Murray, A. et al. / Comparison of three serological tests and an interferon-γ assay for the diagnosis of paratuberculosis in experimentally infected sheep. In: Australian Veterinary Journal. 2000 ; Vol. 78, No. 11. pp. 779-783.