Comparison of the Effects Induced by Plasma Generated Reactive Species and H₂O₂ on Lactate Dehydrogenase (LDH) Enzyme

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Hao Zhang
  • Jie Ma
  • Jie Shen
  • Yan Lan
  • Lili Ding
  • Shulou Qian
  • Cheng Cheng
  • Weidong Xia

Detail(s)

Original languageEnglish
Pages (from-to)2742-2752
Journal / PublicationIEEE Transactions on Plasma Science
Volume46
Issue number8
Online published8 Jun 2018
Publication statusPublished - Aug 2018

Abstract

The influence of reactive species (RS) produced by atmospheric-pressure plasma on lactate dehydrogenase (LDH) enzyme in an aqueous solution is examined, and the long-lived RS in the plasma-treated liquid are probed spectrophotometrically. The concentration of RS increases with treatment time and decreases with storage time. The plasma treatment results in the significant change in viability of LDH. Circular dichroism and dynamic light scattering indicate that the change in the activity systems from plasma-induced modification of the secondary molecular structure as well as polymerization of the peptide chains by the RS produced by the plasma. By comparing the plasma treatment of LDH with the H₂O₂ treatment, the plasma effects are not mediated purely by H₂O₂ and there are instead synergistic effects in the interactions between the plasma and LDH.

Research Area(s)

  • Atmospheric-pressure cold plasma, Helium, Ions, Liquids, molecular structure, Nitrogen, Plasmas, protein inactivation, Proteins, reactive species (RS), synergistic effects.

Citation Format(s)

Comparison of the Effects Induced by Plasma Generated Reactive Species and H₂O₂ on Lactate Dehydrogenase (LDH) Enzyme. / Zhang, Hao; Ma, Jie; Shen, Jie; Lan, Yan; Ding, Lili; Qian, Shulou; Cheng, Cheng; Xia, Weidong; Chu, Paul K.

In: IEEE Transactions on Plasma Science, Vol. 46, No. 8, 08.2018, p. 2742-2752.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review