Comparative transcriptomics of multidrug-resistant Acinetobacter baumannii in response to antibiotic treatments

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

3 Scopus Citations
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Author(s)

  • Hao Qin
  • Norman Wai-Sing Lo
  • Jacky Fong-Chuen Loo
  • Xiao Lin
  • Aldrin Kay-Yuen Yim
  • Stephen Kwok-Wing Tsui
  • Margaret Ip
  • Ting-Fung Chan

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Detail(s)

Original languageEnglish
Article number3515
Journal / PublicationScientific Reports
Volume8
Early online date23 Feb 2018
Publication statusPublished - 2018

Link(s)

Abstract

Multidrug-resistant Acinetobacter baumannii, a major hospital-acquired pathogen, is a serious health threat and poses a great challenge to healthcare providers. Although there have been many genomic studies on the evolution and antibiotic resistance of this species, there have been very limited transcriptome studies on its responses to antibiotics. We conducted a comparative transcriptomic study on 12 strains with different growth rates and antibiotic resistance profiles, including 3 fast-growing pan-drug-resistant strains, under separate treatment with 3 antibiotics, namely amikacin, imipenem, and meropenem. We performed deep sequencing using a strand-specific RNA-sequencing protocol, and used de novo transcriptome assembly to analyze gene expression in the form of polycistronic transcripts. Our results indicated that genes associated with transposable elements generally showed higher levels of expression under antibiotic-treated conditions, and many of these transposon-associated genes have previously been linked to drug resistance. Using co-expressed transposon genes as markers, we further identified and experimentally validated two novel genes of which overexpression conferred significant increases in amikacin resistance. To the best of our knowledge, this study represents the first comparative transcriptomic analysis of multidrug-resistant A. baumannii under different antibiotic treatments, and revealed a new relationship between transposons and antibiotic resistance.

Research Area(s)

Citation Format(s)

Comparative transcriptomics of multidrug-resistant Acinetobacter baumannii in response to antibiotic treatments. / Qin, Hao; Lo, Norman Wai-Sing; Loo, Jacky Fong-Chuen; Lin, Xiao; Yim, Aldrin Kay-Yuen; Tsui, Stephen Kwok-Wing; Lau, Terrence Chi-Kong; Ip, Margaret; Chan, Ting-Fung.

In: Scientific Reports, Vol. 8, 3515, 2018.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

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