Comparative transcriptomic analysis revealed the key pathways responsible for organic sulfur removal by thermophilic bacterium Geobacillus thermoglucosidasius W-2

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

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Author(s)

  • Chenchen Peng
  • Di Huang
  • Yukun Shi
  • Bingling Zhang
  • Linbo Sun
  • Mingchang Li
  • Wei Wang

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)639-650
Journal / PublicationScience of the Total Environment
Volume676
Early online date23 Apr 2019
Publication statusPublished - 1 Aug 2019

Abstract

Biodesulfurization is a promising method to desulfurize sulfur-containing compounds in oil with its unique advantages, such as environment-friendly treatments and moderate reaction conditions. In this study, a thermophilic bacterium Geobacillus thermoglucosidasius W-2 was reported to show nearly 40% and 55% desulfurization rates on heavy oil with 2.81% and 0.46% initial total sulfur content, respectively. Subsequently, comparative transcriptome analysis indicated that several possible key desulfurization-related genes of this strain were found to be differentially up-regulated induced by benzothiophene and dibenzothiophene, respectively. These desulfurization-related genes were considered to conduct key step to convert organic sulfur to inorganic sulfur. Moreover, the characterization of thermophilic alkanesulfonate monooxygenase systems SsuD 1 /SsuE 1 and SsuD 2 /SsuE 2 revealed that the enzymes exhibit considerable thermal and pH stability and wide substrates applicability. These enzymes probably endowed the strain W-2 with the ability to desulfurize oil and eliminate the sulfur-containing surfactants. Thus, this study provides novel alkanesulfonate monooxygenase systems that have the application potential for heavy oil biodesulfurization, oil demulsification and other biocatalytic processes.

Research Area(s)

  • Alkanesulfonate monooxygenase, Biodesulfurization, Demulsification, Dibenzothiophene

Citation Format(s)

Comparative transcriptomic analysis revealed the key pathways responsible for organic sulfur removal by thermophilic bacterium Geobacillus thermoglucosidasius W-2. / Peng, Chenchen; Huang, Di; Shi, Yukun; Zhang, Bingling; Sun, Linbo; Li, Mingchang; Deng, Xin; Wang, Wei.

In: Science of the Total Environment, Vol. 676, 01.08.2019, p. 639-650.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review