Comparative studies of the cellular uptake, subcellular localization, and cytotoxic and phototoxic antitumor properties of ruthenium(II)-porphyrin conjugates with different linkers

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Jing-Xiang Zhang
  • Jun-Wei Zhou
  • Chi-Fai Chan
  • Daniel W. J. Kwong
  • Hoi-Lam Tam
  • Nai-Ki Mak
  • Ka-Leung Wong
  • Wai-Kwok Wong

Detail(s)

Original languageEnglish
Pages (from-to)1623-1638
Journal / PublicationBioconjugate Chemistry
Volume23
Issue number8
Publication statusPublished - 15 Aug 2012

Abstract

Six water-soluble free-base porphyrin-Ru(II) conjugates, 1-3, and Zn(II) porphyrin-Ru(II) conjugates, 4-6, with different linkers between the hydrophobic porphyrin moiety and the hydrophilic Ru(II)-polypyridyl complex, have been synthesized. The linear and two-photon-induced photophysical properties of these conjugates were measured and evaluated for their potential application as dual in vitro imaging and photodynamic therapeutic (PDT) agents. Conjugates 1-3, with their high luminescence and singlet oxygen quantum yields, were selected for further study of their cellular uptake, subcellular localization, and cytotoxic and photocytotoxic (under linear and two-photon excitation) properties using HeLa cells. Conjugate 2, with its hydrophobic phenylethynyl linker, was shown to be highly promising for further development as a bifunctional probe for two-photon (NIR) induced PDT and in vitro imaging. Cellular uptake and subcellular localization properties were shown to be crucial to its PDT efficacy. © 2012 American Chemical Society.

Citation Format(s)

Comparative studies of the cellular uptake, subcellular localization, and cytotoxic and phototoxic antitumor properties of ruthenium(II)-porphyrin conjugates with different linkers. / Zhang, Jing-Xiang; Zhou, Jun-Wei; Chan, Chi-Fai; Lau, Terrence Chi-Kong; Kwong, Daniel W. J.; Tam, Hoi-Lam; Mak, Nai-Ki; Wong, Ka-Leung; Wong, Wai-Kwok.

In: Bioconjugate Chemistry, Vol. 23, No. 8, 15.08.2012, p. 1623-1638.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal