Comparative analysis of outer membrane vesicles from uropathogenic Escherichia coli reveal the role of aromatic amino acids synthesis proteins in motility

LiangZhe Liu (Co-first Author), Carmen Oi Kwan Law (Co-first Author), Qichang Nie (Co-first Author), Hoa Quynh Pham, Haiying Ma, Liang Zhang, Pak Leung Ho, Terrence Chi-Kong Lau*

*Corresponding author for this work

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

1 Citation (Scopus)
91 Downloads (CityUHK Scholars)

Abstract

Uropathogenic Escherichia coli (UPEC) are causative agent that causes urinary tract infections (UTIs) and the recent emergence of multidrug resistance (MDR) of UPEC increases the burden on the community. Recent studies of bacterial outer membrane vesicles (OMV) identified various factors including proteins, nucleic acids, and small molecules which provided inter-cellular communication within the bacterial population. However, the components of UPEC-specific OMVs and their functional role remain unclear. Here, we systematically determined the proteomes of UPEC-OMVs and identified the specific components that provide functions to the recipient bacteria. Based on the functional network of OMVs’ proteomes, a group of signaling peptides was found in all OMVs which provide communication among bacteria. Moreover, we demonstrated that treatment with UPEC-OMVs affected the motility and biofilm formation of the recipient bacteria, and further identified aromatic amino acid (AAA) biosynthesis proteins as the key factors to provide their movement.
Original languageEnglish
Article number151573
JournalInternational Journal of Medical Microbiology
Volume313
Issue number1
Online published6 Jan 2023
DOIs
Publication statusPublished - Jan 2023

Funding

This research was supported by Science, Technology and Innovation Commission of Shenzhen Municipality (grant no. JCYJ201803071238 52823); Research Grants Council, University Grants Committee (grant no. CityU 11101518); and Health and Medical Research Fund, Food and Health Bureau, Hong Kong SAR, China (grant no. 20190932). The graphical abstract was created with BioRender.com.

Research Keywords

  • Aromatic amino acid
  • Biosynthesis proteins
  • Motility
  • Outer membrane vesicles (OMV)
  • Proteomics
  • UPEC

Publisher's Copyright Statement

  • This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/

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