Community dynamics and activity of nirS-harboring denitrifiers in sediments of the Indus River Estuary

Fozia; Yanling Zheng; Lijun Hou; Zongxiao Zhang; Dengzhou Gao; Guoyu Yin; Ping Han; Hongpo Dong; Xia Liang; Yi Yang; Min Liu

doi:10.1016/j.marpolbul.2020.110971

Community dynamics and activity of nirS-harboring denitrifiers in sediments of the Indus River Estuary

Fozia
, Yanling Zheng^*
, Lijun Hou^*
, Zongxiao Zhang
, Dengzhou Gao
, Guoyu Yin
, Ping Han
, Hongpo Dong
, Xia Liang
, Yi Yang
, Min Liu

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

18 Citations (Scopus)

Abstract

Denitrification is an important pathway for reactive nitrogen removal from aquatic ecosystems. In this study, the biodiversity, abundance, and activity of cytochrome cd₁-type nitrate reductase gene (nirS)-harboring denitrifiers in the sediments of the Indus River Estuary were examined by molecular and isotope-tracing techniques. Results showed that the nirS-harboring denitrifier communities showed significant geographical variations along the estuarine salinity gradient. Real-time quantitative PCR showed that the abundance of nirS-harboring denitrifiers ranged from 5.3 × 10⁶ to 2.5 × 10⁸ copies g⁻¹, without significant spatiotemporal variation. The potential rates of denitrification varied from 0.01 to 6.27 μmol N kg⁻¹ h⁻¹ and correlated significantly to TOC and Fe(II) (P < 0.05). On the basis of ¹⁵N isotope-tracing experiments, the denitrification process contributed 18.4–99.4% to the total nitrogen loss in the sediments of the Indus River Estuary. This study provides novel insights into the microbial mechanism of nitrogen removal process in estuarine ecosystems. © 2020 Elsevier Ltd.

Original language	English
Article number	110971
Journal	Marine Pollution Bulletin
Volume	153
Online published	15 Feb 2020
DOIs	https://doi.org/10.1016/j.marpolbul.2020.110971
Publication status	Published - Apr 2020
Externally published	Yes

Funding

This work is funded by National Natural Science Foundation of China (Nos. 41725002, 41671463, 41761144062, 41730646, 41601530, and 41971105). It is also supported by Chinese National Key Programs for Fundamental Research and Development (No. 2016YFA0600904 and 2016YFE0133700), and the Yangtze Delta Estuarine Wetland Station, East China Normal University. Gene sequence data in this paper can be downloaded from National Center for Biotechnology Information (NCBI) under accession SRP230081, and other data can be obtained by sending a written request to the corresponding author.

Research Keywords

Denitrification
nirS gene
Nitrogen
The Indus River Estuary

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title = "Community dynamics and activity of nirS-harboring denitrifiers in sediments of the Indus River Estuary",

abstract = "Denitrification is an important pathway for reactive nitrogen removal from aquatic ecosystems. In this study, the biodiversity, abundance, and activity of cytochrome cd1-type nitrate reductase gene (nirS)-harboring denitrifiers in the sediments of the Indus River Estuary were examined by molecular and isotope-tracing techniques. Results showed that the nirS-harboring denitrifier communities showed significant geographical variations along the estuarine salinity gradient. Real-time quantitative PCR showed that the abundance of nirS-harboring denitrifiers ranged from 5.3 × 106 to 2.5 × 108 copies g−1, without significant spatiotemporal variation. The potential rates of denitrification varied from 0.01 to 6.27 μmol N kg−1 h−1 and correlated significantly to TOC and Fe(II) (P < 0.05). On the basis of 15N isotope-tracing experiments, the denitrification process contributed 18.4–99.4\% to the total nitrogen loss in the sediments of the Indus River Estuary. This study provides novel insights into the microbial mechanism of nitrogen removal process in estuarine ecosystems. {\textcopyright} 2020 Elsevier Ltd.",

keywords = "Denitrification, nirS gene, Nitrogen, The Indus River Estuary",

author = "Fozia and Yanling Zheng and Lijun Hou and Zongxiao Zhang and Dengzhou Gao and Guoyu Yin and Ping Han and Hongpo Dong and Xia Liang and Yi Yang and Min Liu",

year = "2020",

month = apr,

doi = "10.1016/j.marpolbul.2020.110971",

language = "English",

volume = "153",

journal = "Marine Pollution Bulletin",

issn = "0025-326X",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Community dynamics and activity of nirS-harboring denitrifiers in sediments of the Indus River Estuary

AU - Fozia, null

AU - Zheng, Yanling

AU - Hou, Lijun

AU - Zhang, Zongxiao

AU - Gao, Dengzhou

AU - Yin, Guoyu

AU - Han, Ping

AU - Dong, Hongpo

AU - Liang, Xia

AU - Yang, Yi

AU - Liu, Min

PY - 2020/4

Y1 - 2020/4

N2 - Denitrification is an important pathway for reactive nitrogen removal from aquatic ecosystems. In this study, the biodiversity, abundance, and activity of cytochrome cd1-type nitrate reductase gene (nirS)-harboring denitrifiers in the sediments of the Indus River Estuary were examined by molecular and isotope-tracing techniques. Results showed that the nirS-harboring denitrifier communities showed significant geographical variations along the estuarine salinity gradient. Real-time quantitative PCR showed that the abundance of nirS-harboring denitrifiers ranged from 5.3 × 106 to 2.5 × 108 copies g−1, without significant spatiotemporal variation. The potential rates of denitrification varied from 0.01 to 6.27 μmol N kg−1 h−1 and correlated significantly to TOC and Fe(II) (P < 0.05). On the basis of 15N isotope-tracing experiments, the denitrification process contributed 18.4–99.4% to the total nitrogen loss in the sediments of the Indus River Estuary. This study provides novel insights into the microbial mechanism of nitrogen removal process in estuarine ecosystems. © 2020 Elsevier Ltd.

AB - Denitrification is an important pathway for reactive nitrogen removal from aquatic ecosystems. In this study, the biodiversity, abundance, and activity of cytochrome cd1-type nitrate reductase gene (nirS)-harboring denitrifiers in the sediments of the Indus River Estuary were examined by molecular and isotope-tracing techniques. Results showed that the nirS-harboring denitrifier communities showed significant geographical variations along the estuarine salinity gradient. Real-time quantitative PCR showed that the abundance of nirS-harboring denitrifiers ranged from 5.3 × 106 to 2.5 × 108 copies g−1, without significant spatiotemporal variation. The potential rates of denitrification varied from 0.01 to 6.27 μmol N kg−1 h−1 and correlated significantly to TOC and Fe(II) (P < 0.05). On the basis of 15N isotope-tracing experiments, the denitrification process contributed 18.4–99.4% to the total nitrogen loss in the sediments of the Indus River Estuary. This study provides novel insights into the microbial mechanism of nitrogen removal process in estuarine ecosystems. © 2020 Elsevier Ltd.

KW - Denitrification

KW - nirS gene

KW - Nitrogen

KW - The Indus River Estuary

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U2 - 10.1016/j.marpolbul.2020.110971

DO - 10.1016/j.marpolbul.2020.110971

M3 - RGC 21 - Publication in refereed journal

C2 - 32275529

SN - 0025-326X

VL - 153

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

M1 - 110971

ER -

Community dynamics and activity of nirS-harboring denitrifiers in sediments of the Indus River Estuary

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