Combined NK Cell Therapy and Radiation Therapy Exhibit Long-Term Therapeutic and Antimetastatic Effects in a Human Triple Negative Breast Cancer Model

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Kyung Won Kim
  • Jae-UK. Jeong
  • Kyung-Hwa Lee
  • Tung Nguyen Thanh Uong
  • Joon Haeng Rhee
  • Sung-Ja Ahn
  • Sang-Ki Kim
  • Duck Cho
  • Huy Phuoc Quang Nguyen
  • Mee Sun Yoon

Detail(s)

Original languageEnglish
Pages (from-to)115-125
Journal / PublicationInternational Journal of Radiation Oncology Biology Physics
Volume108
Issue number1
Online published10 Oct 2019
Publication statusPublished - 1 Sep 2020

Abstract

Purpose: We investigated whether adoptive cell therapy with ex vivo–activated natural killer (NK) cells enhances the therapeutic efficacy of local tumor radiation therapy (RT) using a human triple-negative breast cancer xenograft model. Methods and Materials: NK cells from healthy donors were expanded ex vivo. MDA-MB-231/Luc-GFP cells were subcutaneously implanted into the thighs of NSG mice. The animals were divided into 4 experimental groups: control, RT, NK, and RT + NK. On day 17 after tumor implantation, tumors from the RT groups were irradiated. The ex vivo–expanded NK cells were intravenously administered twice, on days 17 and 19. Primary and secondary tumors were evaluated using long-term bioluminescence imaging, and histopathology was performed on resected tumor tissue specimens. Results: The luciferase signals of the primary tumors in the RT + NK group were significantly lower than those of comparably sized primary tumors in the RT group. The long-term migration and infiltration of NK cells into the primary tumor sites were significantly higher in RT + NK than in NK mice. Moreover, lymphatic metastasis to the axillary lymph nodes and liver and lung metastases were highly suppressed in the RT + NK group, as demonstrated by BLI and p53 immunohistochemistry. The long-term survival of the RT + NK group was significantly higher than that of the RT or NK groups. Conclusions: Reduction in tumor burden by combining RT and systemic NK cell therapy improved the suppression of primary tumor growth, with efficient NK cell migration and penetration into the primary tumor site. Administered NK cells were maintained in the primary tissue for a significantly longer time in RT + NK group compared with NK group. Both lymphatic spread and distant metastasis to the lungs and liver were effectively suppressed by the combined therapy.

Citation Format(s)

Combined NK Cell Therapy and Radiation Therapy Exhibit Long-Term Therapeutic and Antimetastatic Effects in a Human Triple Negative Breast Cancer Model. / Kim, Kyung Won; Jeong, Jae-UK.; Lee, Kyung-Hwa; Uong, Tung Nguyen Thanh; Rhee, Joon Haeng; Ahn, Sung-Ja; Kim, Sang-Ki; Cho, Duck; Quang Nguyen, Huy Phuoc; Pham, Chanh Tin; Yoon, Mee Sun.

In: International Journal of Radiation Oncology Biology Physics, Vol. 108, No. 1, 01.09.2020, p. 115-125.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review