Colorimetric Detection of Botulinum Neurotoxin Activity Using Gold Nanoparticles
Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45) › 32_Refereed conference paper (with ISBN/ISSN) › peer-review
Author(s)
Detail(s)
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 9th IEEE International Conference on Nano/Molecular Medicine and Engineering |
| Publisher | IEEE Computer Society |
| Pages | 68-71 |
| ISBN (Print) | 9781467396721, 9781467396714 |
| Publication status | Published - Nov 2015 |
Publication series
| Name | |
|---|---|
| ISSN (Print) | 2159-6964 |
| ISSN (Electronic) | 2159-6972 |
Conference
| Title | 9th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2015) |
|---|---|
| Place | United States |
| City | Honolulu |
| Period | 15 - 18 November 2015 |
Link(s)
Abstract
We report a sensitive colorimetric detection of botulinum neurotoxin (BoNT) activity using gold nanoparticles (AuNPs). First, the SNAPtide modified with biotin and cysteine in its two ends, would be cleaved into two short peptides by BoNT activity. The unreacted and cleaved peptides with biotin would be removed by magnetic separation, leaving only cleaved peptides with cysteine end. Next, AuNPs were added to bind with the cleaved peptides via thiol group of cysteine. These AuNPs functionalized with peptides would aggregate and cause a color change by addition of Cu2+. By this method, the detection limit of BoNTs reached to 1 ng/ml (6.67 pM), which is so far the most sensitive colorimetric detection of BoNT activity, providing a simple and instrumentation free approach suitable for resource-poor settings.
Research Area(s)
- botulinum neurotoxin, colorimetric detection, gold nanoparticles
Citation Format(s)
Colorimetric Detection of Botulinum Neurotoxin Activity Using Gold Nanoparticles. / Chen, Shan; Chu, Lok Ting; Chen, Ting-Hsuan.
Proceedings of the 9th IEEE International Conference on Nano/Molecular Medicine and Engineering. IEEE Computer Society, 2015. p. 68-71 7492504.Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45) › 32_Refereed conference paper (with ISBN/ISSN) › peer-review
