Colorimetric Detection of Botulinum Neurotoxin Activity Using Gold Nanoparticles

Shan Chen; Lok Ting Chu; Ting-Hsuan Chen

doi:10.1109/NANOMED.2015.7492504

Colorimetric Detection of Botulinum Neurotoxin Activity Using Gold Nanoparticles

Shan Chen, Lok Ting Chu, Ting-Hsuan Chen^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

We report a sensitive colorimetric detection of botulinum neurotoxin (BoNT) activity using gold nanoparticles (AuNPs). First, the SNAPtide modified with biotin and cysteine in its two ends, would be cleaved into two short peptides by BoNT activity. The unreacted and cleaved peptides with biotin would be removed by magnetic separation, leaving only cleaved peptides with cysteine end. Next, AuNPs were added to bind with the cleaved peptides via thiol group of cysteine. These AuNPs functionalized with peptides would aggregate and cause a color change by addition of Cu²⁺. By this method, the detection limit of BoNTs reached to 1 ng/ml (6.67 pM), which is so far the most sensitive colorimetric detection of BoNT activity, providing a simple and instrumentation free approach suitable for resource-poor settings.

Original language	English
Title of host publication	Proceedings of the 9th IEEE International Conference on Nano/Molecular Medicine and Engineering
Publisher	IEEE Computer Society
Pages	68-71
ISBN (Print)	9781467396721, 9781467396714
DOIs	https://doi.org/10.1109/NANOMED.2015.7492504
Publication status	Published - Nov 2015
Event	9th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2015) - Honolulu, United States Duration: 15 Nov 2015 → 18 Nov 2015

Publication series

Name
ISSN (Print)	2159-6964
ISSN (Electronic)	2159-6972

Conference

Conference	9th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2015)
Place	United States
City	Honolulu
Period	15/11/15 → 18/11/15

Research Keywords

botulinum neurotoxin
colorimetric detection
gold nanoparticles

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10.1109/NANOMED.2015.7492504

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Cite this

@inproceedings{3d50f11366b4489b9d6ed8ebecbc178a,

title = "Colorimetric Detection of Botulinum Neurotoxin Activity Using Gold Nanoparticles",

abstract = "We report a sensitive colorimetric detection of botulinum neurotoxin (BoNT) activity using gold nanoparticles (AuNPs). First, the SNAPtide modified with biotin and cysteine in its two ends, would be cleaved into two short peptides by BoNT activity. The unreacted and cleaved peptides with biotin would be removed by magnetic separation, leaving only cleaved peptides with cysteine end. Next, AuNPs were added to bind with the cleaved peptides via thiol group of cysteine. These AuNPs functionalized with peptides would aggregate and cause a color change by addition of Cu2+. By this method, the detection limit of BoNTs reached to 1 ng/ml (6.67 pM), which is so far the most sensitive colorimetric detection of BoNT activity, providing a simple and instrumentation free approach suitable for resource-poor settings.",

keywords = "botulinum neurotoxin, colorimetric detection, gold nanoparticles",

author = "Shan Chen and Chu, {Lok Ting} and Ting-Hsuan Chen",

year = "2015",

month = nov,

doi = "10.1109/NANOMED.2015.7492504",

language = "English",

isbn = "9781467396721",

publisher = "IEEE Computer Society",

pages = "68--71",

booktitle = "Proceedings of the 9th IEEE International Conference on Nano/Molecular Medicine and Engineering",

address = "United States",

note = "9th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2015) ; Conference date: 15-11-2015 Through 18-11-2015",

}

Chen, S , Chu, LT & Chen, T-H 2015, Colorimetric Detection of Botulinum Neurotoxin Activity Using Gold Nanoparticles. in Proceedings of the 9th IEEE International Conference on Nano/Molecular Medicine and Engineering., 7492504, IEEE Computer Society, pp. 68-71, 9th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2015), Honolulu, Hawaii, United States, 15/11/15. https://doi.org/10.1109/NANOMED.2015.7492504

Colorimetric Detection of Botulinum Neurotoxin Activity Using Gold Nanoparticles. / Chen, Shan ; Chu, Lok Ting ; Chen, Ting-Hsuan.
Proceedings of the 9th IEEE International Conference on Nano/Molecular Medicine and Engineering. IEEE Computer Society, 2015. p. 68-71 7492504.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Colorimetric Detection of Botulinum Neurotoxin Activity Using Gold Nanoparticles

AU - Chen, Shan

AU - Chu, Lok Ting

AU - Chen, Ting-Hsuan

PY - 2015/11

Y1 - 2015/11

N2 - We report a sensitive colorimetric detection of botulinum neurotoxin (BoNT) activity using gold nanoparticles (AuNPs). First, the SNAPtide modified with biotin and cysteine in its two ends, would be cleaved into two short peptides by BoNT activity. The unreacted and cleaved peptides with biotin would be removed by magnetic separation, leaving only cleaved peptides with cysteine end. Next, AuNPs were added to bind with the cleaved peptides via thiol group of cysteine. These AuNPs functionalized with peptides would aggregate and cause a color change by addition of Cu2+. By this method, the detection limit of BoNTs reached to 1 ng/ml (6.67 pM), which is so far the most sensitive colorimetric detection of BoNT activity, providing a simple and instrumentation free approach suitable for resource-poor settings.

AB - We report a sensitive colorimetric detection of botulinum neurotoxin (BoNT) activity using gold nanoparticles (AuNPs). First, the SNAPtide modified with biotin and cysteine in its two ends, would be cleaved into two short peptides by BoNT activity. The unreacted and cleaved peptides with biotin would be removed by magnetic separation, leaving only cleaved peptides with cysteine end. Next, AuNPs were added to bind with the cleaved peptides via thiol group of cysteine. These AuNPs functionalized with peptides would aggregate and cause a color change by addition of Cu2+. By this method, the detection limit of BoNTs reached to 1 ng/ml (6.67 pM), which is so far the most sensitive colorimetric detection of BoNT activity, providing a simple and instrumentation free approach suitable for resource-poor settings.

KW - botulinum neurotoxin

KW - colorimetric detection

KW - gold nanoparticles

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U2 - 10.1109/NANOMED.2015.7492504

DO - 10.1109/NANOMED.2015.7492504

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781467396721

SN - 9781467396714

SP - 68

EP - 71

BT - Proceedings of the 9th IEEE International Conference on Nano/Molecular Medicine and Engineering

PB - IEEE Computer Society

T2 - 9th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2015)

Y2 - 15 November 2015 through 18 November 2015

ER -

Colorimetric Detection of Botulinum Neurotoxin Activity Using Gold Nanoparticles

Abstract

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The Development of Biosensors Using Gold Nanoparticles as Sensing Platform

Cite this

Colorimetric Detection of Botulinum Neurotoxin Activity Using Gold Nanoparticles

Abstract

Publication series

Conference

Research Keywords

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Student theses

The Development of Biosensors Using Gold Nanoparticles as Sensing Platform

Cite this